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WB analysis of human heart using 13349-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13349-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13349-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13349-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13349-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13349-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 13349-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 13349-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 40x lens).

FBLIM1 Polyclonal antibody

Name: FBLIM1 Polyclonal antibody
Brand: Proteintech
SKU: 13349-1-AP

FBLIM1 Polyclonal Antibody for IHC, ELISA

Cat No. 13349-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Synonyms

CAL, FBLIM1, FBLP 1, FBLP1, filamin binding LIM protein 1, MIG2 interacting protein, Migfilin, RP11 169K16.5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in

human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 1 publications below

Product Information

13349-1-AP targets FBLIM1 in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3907 Product name: Recombinant human FBLIM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC019895 Sequence: MASKPEKRVASSVFITLAPPRRDVAVAEEVRQAVCEARRGRPWEAPAPMKTPEAGLAGRPSPWTTPGRAAATVPAAPMQLFNGGCPPPPPVLDGEDVLPDLDLLPPPPPPPPVLLPSEEEAPAPMGASLIADLEQLHLSPPPPPPQAPAEGPSVQPGPLRPMEEELPPPPAEPVEKGASTDICAFCHKTVFPRELAVEAMKRQYHAQCFTCRTCRRQLAGQSFYQKDGRPLCEPCYQDTLERCGKCGEVVRDHIIRALGQAFHPSCFTCVTCARCIGDESFALGSQNEVYCLDDFYRKFAPVCSICENPIIPRDGKDAFKIECMGRNFHENCYRCEDCRILLSVEPTDQGCYPLNNHLFCKPCHVKRSAAGCC Predict reactive species
Full Name	filamin binding LIM protein 1
Calculated Molecular Weight	373 aa, 41 kDa
Observed Molecular Weight	45-48 kDa
GenBank Accession Number	BC019895
Gene Symbol	FBLIM1
Gene ID (NCBI)	54751
RRID	AB_2262903
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WUP2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBLIM1, also known as CAL, Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. FBLIM1 is a protein found in cell-cell and cell-ECM connections where it co-localizes with FLNA/C and FLNB. FBLIM1 was found to bind directly to FLNA/C and to be an important regulator of cell shape and motility. FBLIM1 exerts its influence on cellular functions by interacting with various binding partners; FLN via its N- terminal domain, VASP and Src via its proline-rich region, and kindlin-2 and the cardiac transcription factor, CSX/NKX2-5 via its C-terminal LIM domains. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. FBLIM1 serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. It is associated with actin stress fiber at cell-ECM focal adhesion sites. FBLP-1A and FBLP-1B are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. FBLP-1 is localized at actin stress fibers. FBLIM1 is implicated in cell shape modulation (spreading) and motility. FBLIM1 participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. It may also regulate the assembly of filamin-containing signaling complexes that control actin assembly. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. This antibody is a rabbit polyclonal antibody raised against full length human FBLIM1 antigen.