FBLIM1 Polyclonal antibody

FBLIM1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13349-1-AP

Synonyms

CAL, FBLIM1, FBLP 1, FBLP1, filamin binding LIM protein 1, MIG2 interacting protein, Migfilin, RP11 169K16.5

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Validation Data Gallery

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  WB analysis of HEK-293 using 13349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13349-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13349-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13349-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13349-1-AP (FBLIM1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 13349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 13349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13349-1-AP (FBLIM1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13349-1-AP targets FBLIM1 in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBLIM1 fusion protein Ag3907
• Full Name: filamin binding LIM protein 1
• Calculated Molecular Weight: 373 aa, 41 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC019895
• Gene Symbol: FBLIM1
• Gene ID (NCBI): 54751
• RRID: AB_2262903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBLIM1, also known as CAL, Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. FBLIM1 is a protein found in cell-cell and cell-ECM connections where it co-localizes with FLNA/C and FLNB. FBLIM1 was found to bind directly to FLNA/C and to be an important regulator of cell shape and motility. FBLIM1 exerts its influence on cellular functions by interacting with various binding partners; FLN via its N- terminal domain, VASP and Src via its proline-rich region, and kindlin-2 and the cardiac transcription factor, CSX/NKX2-5 via its C-terminal LIM domains. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. FBLIM1 serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. It is associated with actin stress fiber at cell-ECM focal adhesion sites. FBLP-1A and FBLP-1B are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. FBLP-1 is localized at actin stress fibers. FBLIM1 is implicated in cell shape modulation (spreading) and motility. FBLIM1 participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. It may also regulate the assembly of filamin-containing signaling complexes that control actin assembly. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. This antibody is a rabbit polyclonal antibody raised against full length human FBLIM1 antigen.

Publications

Species	Application	Title
human	IF	J Cell Sci; Functional differences between kindlin-1 and kindlin-2 in keratinocytes.; Authors - Bandyopadhyay Aditi A