FBXL21 Polyclonal antibody

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  IHC staining of mouse heart using 21437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21437-1-AP (FBXL21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21437-1-AP (FBXL21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 21437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21437-1-AP (FBXL21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 21437-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using FBXL21 antibody (21437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FBXL21 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 21437-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

F box/LRR repeat protein 21, F box/LRR repeat protein 3B, Fbl21, FBL3, FBL3B

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Tested Applications

• Positive IHC detected in: mouse heart tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21437-1-AP targets FBXL21 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXL21 fusion protein Ag16049
• Full Name: F-box and leucine-rich repeat protein 21
• Calculated Molecular Weight: 434 aa, 49 kDa
• GenBank Accession Number: BC106753
• Gene Symbol: FBXL21
• Gene ID (NCBI): 26223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3KPF8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXL21 (F-box and leucine-rich repeat protein 21) is a member of the F-box protein family, which plays a crucial role in regulating protein degradation through the ubiquitin-proteasome system. FBXL21 has been identified as a regulator in skeletal muscle. It mediates the rhythmic degradation of sarcomere proteins such as TCAP (titin cap) and MYOZ1 (Myozenin1) in conjunction with GSK-3β (PMID: 36574402).