FBXO21 Polyclonal antibody

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  IP experiment of rat brain using 17648-1-AP

  IP result of anti-FBXO21 (IP:17648-1-AP, 4ug; Detection:17648-1-AP 1:500) with rat brain tissue lysate 1720 ug.

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  IP experiment of A549 using 17648-1-AP

  IP result of anti-FBXO21 (IP:17648-1-AP, 4ug; Detection:17648-1-AP 1:500) with A549 cells lysate 880 ug.

FBXO21 Polyclonal Antibody for IP, ELISA

Cat No. 17648-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IP, ELISA

Synonyms

F box only protein 21, F box protein 21, F-box only protein 21, FBX21, FBXO 21

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Tested Applications

• Positive IP detected in: A549 cells, rat brain tissue

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17648-1-AP targets FBXO21 in IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXO21 fusion protein Ag11882
• Full Name: F-box protein 21
• Calculated Molecular Weight: 537 aa, 63 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC034045
• Gene Symbol: FBXO21
• Gene ID (NCBI): 23014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94952
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO21 (F-box protein 21) is a member of the F-box protein family and serves as the substrate recognition component of the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex. In AML cells, FBXO21 can ubiquitinate p85α, a regulatory subunit of the PI3K pathway, leading to its degradation. This reduces PI3K signaling, promotes p85α dimerization, and activates ERK, thereby influencing cell proliferation and survival. Silencing FBXO21 in AML cells induces differentiation, inhibits tumor progression, and increases sensitivity to chemotherapeutic agents. FBXO21 can regulate immune responses by ubiquitinating and degrading proteins like ASK1 through pro-inflammatory cytokine pathways. It can also ubiquitinate p85α, affecting CXCL10 expression and modulating immune cell infiltration and anti-tumor activity. In renal cell carcinoma, overexpression of FBXO21 significantly suppresses ccRCC cell proliferation and metastasis both in vitro and in vivo. It may be related to the stromal score and the infiltration levels of immune cells associated with prognosis. Additionally, FBXO21 overexpression increases the expression of key molecules in the CREB pathway.