|Positive WB detected in||THP-1 cells, U-937 cells, HL-60 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
27563-1-AP targets CD64 in WB applications and shows reactivity with Human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD64 fusion protein Ag26727|
|Full Name||Fc fragment of IgG, high affinity Ia, receptor (CD64)|
|Calculated molecular weight||374 aa, 43 kDa|
|Observed molecular weight||65-70 kDa, 43-45 kDa|
|GenBank accession number||BC032634|
|Gene ID (NCBI)||2209|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.