FGFR1 Monoclonal antibody

FGFR1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

10F12E7

Cat no : 60325-1-Ig

Synonyms

bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr



Tested Applications

Positive WB detected inMCF-7 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60325-1-Ig targets FGFR1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen FGFR1 fusion protein Ag3646
Full Name fibroblast growth factor receptor 1
Calculated molecular weight 820 aa, 91 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC018128
Gene symbol FGFR1
Gene ID (NCBI) 2260
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processed including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. Alternatively spliced variants which encode different protein isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for FGFR1 antibody 60325-1-IgDownload protocol
IHC protocol for FGFR1 antibody 60325-1-IgDownload protocol
FC protocol for FGFR1 antibody 60325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

CNS Neurosci Ther

Dl-3-N-butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats.

Authors - Pan-Ting Zhou
ratWB

Pain Physician

Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells.

Authors - Liang Zhang
ratWB

Am J Transl Res

Synergistic protection of tetramethylpyrazine phosphate and borneol on brain microvascular endothelium cells injured by hypoxia.

Authors - Bin Yu
humanWB,IHC

Biochim Biophys Acta

Long non-coding RNA PVT1 promotes malignancy in human endometrial carcinoma cells through negative regulation of miR-195-5p.

Authors - Fanfei Kong
humanWB

Genet Mol Res

Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5.

Authors - T Wang
mouseWB

Mol Immunol

Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.

Authors - Libo Hou