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FGFR1 Antibody 4 Publications

Mouse Monoclonal| Catalog number: 60325-1-Ig |CloneNo.: 10F12E7

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Con: 168 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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Images See all

FGFR1 Antibody WB MCF-7 cells 60325-1-Ig

MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

FGFR1 Antibody IHC human breast cancer tissue 60325-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig( FGFR1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

FGFR1 Antibody IHC human breast cancer tissue 60325-1-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig( FGFR1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

FGFR1 Antibody FC K-562 cells 60325-1-Ig

1X10^6 K-562 cells were stained with 0.2ug FGFR1 antibody (60325-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse
Positive WB detected in:
MCF-7 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells, 3T3-L1 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
K-562 cells
Recommended dilution:
WB : 1:1000-1:3000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2c
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FGFR1 fusion protein Ag3646
Full name:
fibroblast growth factor receptor 1
Calculated molecular weight:
820aa,91 kDa
Observed molecular weight:
91 kDa
GenBank accession number:
BC018128
Gene ID (NCBI):
2260
Gene symbol
FGFR1
Synonyms
bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr

Background

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processed including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. Alternatively spliced variants which encode different protein isoforms have been described.


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