3T3-L1 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig( FGFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig( FGFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Y79 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig( FGFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig( FGFR1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig( FGFR1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

1X10^6 K-562 cells were stained with 0.2ug FGFR1 antibody (60325-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.