FGFR1 Monoclonal antibody

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  WB analysis of MCF-7 using 60325-1-Ig

  MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of ACHN using 60325-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed ACHN cells using FGFR1 antibody (60325-1-Ig, Clone: 10F12E7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of K-562 using 60325-1-Ig

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug FGFR1 Monoclonal antibody (60325-1-Ig, Clone:10F12E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FGFR1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 60325-1-Ig

Clone No.10F12E7

Publications(39)

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.7.10.1, CEK, CD331, bFGF-R-1, BFGFR

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Tested Applications

• Positive WB detected in: MCF-7 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: ACHN cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 35 publications below
• IHC: See 11 publications below
• IF: See 2 publications below

Product Information

60325-1-Ig targets FGFR1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGFR1 fusion protein Ag3646
• Full Name: fibroblast growth factor receptor 1
• Calculated Molecular Weight: 820 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC018128
• Gene Symbol: FGFR1
• Gene ID (NCBI): 2260
• RRID: AB_2881435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11362
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
	IHC	ACS Appl Mater Interfaces; Hydrogel Loaded with Peptide-Containing Nanocomplexes: Symphonic Cooperation of Photothermal Antimicrobial Nanoparticles and Prohealing Peptides for the Treatment of Infected Wounds; Authors - Qiuye Jia
human	WB	Theranostics; Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.; Authors - Meng Wang
human	WB,IHC	J Transl Med; A novel intronic circular RNA circFGFR1int2 up-regulates FGFR1 by recruiting transcriptional activators P65/FUS and suppressing miR-4687-5p to promote prostate cancer progression; Authors - Ruyue Wang
human	WB	Oxid Med Cell Longev; DJ-1/FGFR-1 Signaling Pathway Contributes to Sorafenib Resistance in Hepatocellular Carcinoma.; Authors - Xin Chen
human	WB	Int J Mol Sci; The FGFR Family Inhibitor AZD4547 Exerts an Antitumor Effect in Ovarian Cancer Cells.; Authors - Yu Ran Na