FGFR1 Monoclonal antibody
FGFR1 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2c
Reactivity
human
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
10F12E7
Cat no : 60325-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 13 publications below |
| IHC | See 2 publications below |
Product Information
60325-1-Ig targets FGFR1 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FGFR1 fusion protein Ag3646 |
| Full Name | fibroblast growth factor receptor 1 |
| Calculated molecular weight | 820 aa, 91 kDa |
| Observed molecular weight | 91 kDa |
| GenBank accession number | BC018128 |
| Gene symbol | FGFR1 |
| Gene ID (NCBI) | 2260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processed including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. Alternatively spliced variants which encode different protein isoforms have been described.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FGFR1 antibody 60325-1-Ig | Download protocol |
| IHC protocol for FGFR1 antibody 60325-1-Ig | Download protocol |
| FC protocol for FGFR1 antibody 60325-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther Dl-3-N-butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats. | ||
Pain Physician Toxicity Effects of Methylene Blue on Rat Intervertebral Disc Annulus Fibrosus Cells. | ||
Am J Transl Res Synergistic protection of tetramethylpyrazine phosphate and borneol on brain microvascular endothelium cells injured by hypoxia. | ||
Biochim Biophys Acta Long non-coding RNA PVT1 promotes malignancy in human endometrial carcinoma cells through negative regulation of miR-195-5p. | ||
Gastroenterol Rep (Oxf) Upregulated insulin receptor tyrosine kinase substrate promotes the proliferation of colorectal cancer cells via the bFGF/AKT signaling pathway. | ||
Genet Mol Res Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5. |
