FGFR1 Monoclonal antibody

FGFR1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

21

CloneNo.

10F12E7

Cat no : 60325-1-Ig

Synonyms

bFGF R 1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR1, FLG, FLT 2, FLT2, Fms like tyrosine kinase 2, HBGFR, KAL2, N SAM, OGD, Proto oncogene c Fgr

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Validation Data Gallery

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  WB analysis of MCF-7 using 60325-1-Ig

  MCF-7, HT-1080, HUVEC, U2OS, Y79, and K-562 cells were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60325-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60325-1-Ig (FGFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60325-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60325-1-Ig (FGFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of K-562 using 60325-1-Ig

  1X10^6 K-562 cells were stained with 0.2ug FGFR1 antibody (60325-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: MCF-7 cells, NIH/3T3 cells, HT-1080 cells, HUVEC cells, U2OS cells, Y79 cells, K-562 cells, 4T1 cells, HSC-T6 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

60325-1-Ig targets FGFR1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGFR1 fusion protein Ag3646
• Full Name: fibroblast growth factor receptor 1
• Calculated molecular weight: 820 aa, 91 kDa
• Observed molecular weight: 91 kDa
• GenBank accession number: BC018128
• Gene symbol: FGFR1
• Gene ID (NCBI): 2260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processed including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome. Alternatively spliced variants which encode different protein isoforms have been described.

Publications

Species	Application	Title
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human,mouse	WB	Theranostics; Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.; Authors - Meng Wang
mouse	WB	J Cell Physiol; Brivanib, a multitargeted small-molecule tyrosine kinase inhibitor, suppresses laser-induced CNV in a mouse model of neovascular AMD.; Authors - Lele Li
human	WB	Aging (Albany NY); PMID: 32202509; Authors - Sen Hong
human	WB	Front Oncol; LncRNA SNHG1 Promotes the Progression of Pancreatic Cancer by Regulating FGFR1 Expression via Competitively Binding to miR-497.; Authors - Shihong Chen
human	WB,IHC	Sci Rep; The expression and function of miR-424 in infantile skin hemangioma and its mechanism.; Authors - Lili Yang

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