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FKBP52 Polyclonal antibody

FKBP52 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10655-1-AP

Synonyms

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase



Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10655-1-AP targets FKBP52 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP52 fusion protein Ag1019
Full Name FK506 binding protein 4, 59kDa
Calculated molecular weight 59 kDa
Observed molecular weight 56-59 kDa
GenBank accession numberBC007924
Gene symbol FKBP4
Gene ID (NCBI) 2288
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

Protocols

Product Specific Protocols
WB protocol for FKBP52 antibody 10655-1-APDownload protocol
IHC protocol for FKBP52 antibody 10655-1-APDownload protocol
IF protocol for FKBP52 antibody 10655-1-APDownload protocol
IP protocol for FKBP52 antibody 10655-1-APDownload protocol
FC protocol for FKBP52 antibody 10655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Oncoscience

The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.

Authors - Dhanushka Hewa Bostanthirige
chickenWB

Proteome Sci

Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers.

Authors - Chun-Yan Wu
humanWB

Biol Trace Elem Res

miR-378d is Involved in the Regulation of Apoptosis and Autophagy of and E2 Secretion from Cultured Ovarian Granular Cells Treated by Sodium Fluoride.

Authors - Qun Chen
humanIHC

J Neurovirol

Increased cortical expression of FK506 binding protein-51 in HIV-associated neurocognitive disorders.

Authors - Soontornniyomkij Virawudh V
mouseIHC

Age (Dordr)

Short-term recognition memory impairment is associated with decreased expression of FK506 binding protein 51 in the aged mouse brain.

Authors - Soontornniyomkij Virawudh V
mouseWB

RNA

The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.

Authors - Martinez Natalia J NJ
  • KD Validated