FKBP52 Polyclonal antibody

• Featured Product
• KD/KO Validated

FKBP52 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10655-1-AP

Synonyms

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

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Validation Data Gallery

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  WB analysis using 10655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10655-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10655-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10655-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10655-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10655-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10655-1-AP

  IP result of anti-FKBP52 (IP:10655-1-AP, 4ug; Detection:10655-1-AP 1:20000) with HeLa cells lysate 1360 ug.

• 

  IHC staining of human prostate cancer using 10655-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10655-1-AP (FKBP52 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of MCF-7 using 10655-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FKBP52 antibody (10655-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10655-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug FKBP52 antibody (10655-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Product Information

10655-1-AP targets FKBP52 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP52 fusion protein Ag1019
• Full Name: FK506 binding protein 4, 59kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007924
• Gene Symbol: FKBP4
• Gene ID (NCBI): 2288
• RRID: AB_2246853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

Publications

Species	Application	Title
human	WB	Theranostics; FKBP4 connects mTORC2 and PI3K to activate the PDK1/Akt-dependent cell proliferation signaling in breast cancer.; Authors - Alain Mangé ; KD Validated
human	WB,IHC	Clin Cancer Res; Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women.; Authors - Desmetz Caroline C
human	WB，IP	Mol Oncol; FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells.; Authors - Keisuke Maeda ; KD Validated
human	WB	Int J Mol Sci; Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations; Authors - Rika Kojima
human	WB	J Cell Physiol; Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.; Authors - Vishwanatha K S Rao
mouse	WB	RNA; The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.; Authors - Martinez Natalia J NJ ; KD Validated