FLI1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85931-1-RR (same clone as 85931-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85931-1-RR (FLI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  Sample test of MP02198-1

  The mean FLI1 concentration was determined to be 303.2 ng/mL in Jurkat cell extract based on a 1.9 mg/mL extract load.

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  IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 85931-1-RR (same clone as 85931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85931-1-RR (FLI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IF Staining of HEL using 85931-1-RR (same clone as 85931-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85931-1-RR (same clone as 85931-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using FLI1 antibody (85931-1-RR, Clone: 250294F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85931-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02198-1

  Cytometric bead array standard curve of MP02198-1, FLI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85931-3-PBS. Detection antibody: 85931-1-PBS. Standard: Ag36169. Range: 0.781-100 ng/mL.

FLI1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 85931-1-PBS

Clone No.250294F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1

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Product Information

85931-1-PBS targets FLI1 as part of a matched antibody pair:

MP02198-1: 85931-3-PBS capture and 85931-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FLI1 fusion protein Ag36169
• Full Name: Friend leukemia virus integration 1
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC010115
• Gene Symbol: FLI1
• Gene ID (NCBI): 2313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01543
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown.