VEGFR-1/FLT-1 Polyclonal antibody

VEGFR-1/FLT-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13687-1-AP

Synonyms

FLT, FLT 1, FLT1, FLT-1, Fms like tyrosine kinase 1, FRT, sflt1, Tyrosine protein kinase FRT, VEGF receptor 1, VEGFR 1, VEGFR1, VEGFR-1, VEGFR-1/FLT-1



Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, mouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13687-1-AP targets VEGFR-1/FLT-1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFR-1/FLT-1 fusion protein Ag4558
Full Name fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
Calculated molecular weight 1338 aa, 151 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC039007
Gene symbol FLT1
Gene ID (NCBI) 2321
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short souble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)

Protocols

Product Specific Protocols
WB protocol for VEGFR-1/FLT-1 antibody 13687-1-APDownload protocol
IHC protocol for VEGFR-1/FLT-1 antibody 13687-1-APDownload protocol
IF protocol for VEGFR-1/FLT-1 antibody 13687-1-APDownload protocol
IP protocol for VEGFR-1/FLT-1 antibody 13687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cells

Heparanase released from mesenchymal stem cells activates integrin beta1/HIF-2alpha/Flk-1 signaling and promotes endothelial cell migration and angiogenesis.

Authors - Xinyang Hu
mouseWB

Clin Sci (Lond)

Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.

Authors - Mengqiu Wu
humanWB

Stem Cell Res Ther

Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.

Authors - Xuemei Fan
humanWB, IF, IHC

Front Oncol

IL-1β Promotes Vasculogenic Mimicry of Breast Cancer Cells Through p38/MAPK and PI3K/Akt Signaling Pathways.

Authors - Muhammad Azhar Nisar
humanWB

Biochem Pharmacol

RhTyrRS (Y341A), a novel human tyrosyl-tRNA synthetase mutant, stimulates thrombopoiesis through activation of the VEGF-R II/NF-κB pathway.

Authors - Yun Shi
DeerWB

Stem Cell Res Ther

Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration.

Authors - Wei Zhang