Back

VEGFR-1/FLT-1 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 13687-1-AP

Be the first to review this product

-_-

Freight/Packing

Con: 39 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Related Primary Antibodies
Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

VEGFR-1/FLT-1 Antibody WB HEK-293 cells 13687-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13687-1-AP(VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

VEGFR-1/FLT-1 Antibody IP A549 cells 13687-1-AP

IP Result of anti-VEGFR-1/FLT-1 (IP:13687-1-AP, 5ug; Detection:13687-1-AP 1:500) with A549 cells lysate 3300ug.

VEGFR-1/FLT-1 Antibody IF HeLa cells 13687-1-AP

Immunofluorescent analysis of HeLa cells using 13687-1-AP(VEGFR-1/FLT-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

VEGFR-1/FLT-1 Antibody FC HUVEC cells 13687-1-AP

1X10^6 HUVEC cells were stained with 0.2ug VEGFR-1/FLT-1 antibody (13687-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, deer
Positive WB detected in:
HEK-293 cells, human placenta tissue, mouse lung tissue
Positive IP detected in:
A549 cells
Positive IF detected in:
HeLa cells
Positive FC detected in:
HUVEC cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
VEGFR-1/FLT-1 fusion protein Ag4558
Full name:
fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
Calculated molecular weight:
1338aa,151 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
BC039007
Gene ID (NCBI):
2321
Gene symbol
FLT1
Synonyms
FLT, FLT 1, FLT1, FLT-1, Fms like tyrosine kinase 1, FRT, sflt1, Tyrosine protein kinase FRT, VEGF receptor 1, VEGFR 1, VEGFR1, VEGFR-1

Background

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short souble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)


Back
to top