Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human placenta tissue, mouse lung tissue|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13687-1-AP targets VEGFR-1/FLT-1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Deer|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VEGFR-1/FLT-1 fusion protein Ag4558|
|Full Name||fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)|
|Calculated molecular weight||1338 aa, 151 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC039007|
|Gene ID (NCBI)||2321|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short souble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)
|Product Specific Protocols|
|WB protocol for VEGFR-1/FLT-1 antibody 13687-1-AP||Download protocol|
|IHC protocol for VEGFR-1/FLT-1 antibody 13687-1-AP||Download protocol|
|IF protocol for VEGFR-1/FLT-1 antibody 13687-1-AP||Download protocol|
|IP protocol for VEGFR-1/FLT-1 antibody 13687-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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