VEGFR-1/FLT-1 Polyclonal antibody

VEGFR-1/FLT-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 13687-1-AP

Synonyms

FLT, FLT 1, FLT1, FLT-1, Fms like tyrosine kinase 1, FRT, sflt1, Tyrosine protein kinase FRT, VEGF receptor 1, VEGFR 1, VEGFR1, VEGFR-1, VEGFR-1/FLT-1

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Validation Data Gallery

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  WB analysis of HEK-293 using 13687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13687-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13687-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 13687-1-AP

  IP Result of anti-VEGFR-1/FLT-1 (IP:13687-1-AP, 5ug; Detection:13687-1-AP 1:500) with A549 cells lysate 3300ug.

• 

  IHC staining of human renal cell carcinoma using 13687-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13687-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13687-1-AP

  Immunofluorescent analysis of HeLa cells using 13687-1-AP (VEGFR-1/FLT-1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HUVEC using 13687-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug VEGFR-1/FLT-1 antibody (13687-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue, mouse lung tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13687-1-AP targets VEGFR-1/FLT-1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VEGFR-1/FLT-1 fusion protein Ag4558
• Full Name: fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
• Calculated molecular weight: 1338 aa, 151 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC039007
• Gene symbol: FLT1
• Gene ID (NCBI): 2321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Two isoforms of VEGFR-1 exist, a full-length transmembrane form and a short souble form (sVEGFR-1) consisting of only the extracellular ligand-binding domain. (PMID: 12778165; 17109193; 8806634)

Publications

Species	Application	Title
human	WB,IP	Placenta; Tumor necrosis factor-α regulates angiogenesis of BeWo cells via synergy of PlGF/VEGFR1 and VEGF-A/VEGFR2 axes.; Authors - Kei Tanaka
human	WB	BMC Pregnancy Childbirth; Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.; Authors - Xiaotong Sun
	IHC	Int J Biochem Cell Biol; Exosomes from hypoxia-treated human adipose-derived mesenchymal stem cells enhance angiogenesis through VEGF/VEGF-R.; Authors - Yudi Han
human	WB	Int J Biol Macromol; Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2.; Authors - Hui-Ying Hu
human	IHC	Cell Death Dis; Decreased ZNF750 promotes angiogenesis in a paracrine manner via activating DANCR/miR-4707-3p/FOXC2 axis in esophageal squamous cell carcinoma.; Authors - Yanghui Bi
rat	WB	ACS Appl Mater Interfaces; Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.; Authors - Lin Huang

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