VEGFR3/FLT4 Polyclonal antibody

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  WB analysis of mouse spleen using 20712-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20712-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20712-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20712-1-AP (VEGFR3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

VEGFR3/FLT4 Polyclonal Antibody for WB, ELISA

Cat No. 20712-1-AP

Publications(15)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

FLT4, Fms like tyrosine kinase 4, FLT41, FLT-4, FLT 4

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Tested Applications

• Positive WB detected in: mouse spleen tissue, PC-3 cells, HeLa cells, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

20712-1-AP targets VEGFR3/FLT4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: fms-related tyrosine kinase 4
• Calculated Molecular Weight: 153 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: NM_002020
• Gene Symbol: FLT4
• Gene ID (NCBI): 2324
• RRID: AB_10733474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35916
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 cause lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma. VEGFR-3 is a highly glycosylated protein and migrates as bands with different molecular weights: an ~175 kDa precursor, an ~195 kDa mature form, an ~140 kDa non-glycosylated backbone, and a form that appears to be partially cleaved proteolytically in the extracellular domain to produce an ~125 kDa band(PMID: 15389531).

Publications

Species	Application	Title
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
human	IHC	Front Oncol; The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis.; Authors - Shuai-Jun Xu
Deer	WB	Stem Cell Res Ther; Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration.; Authors - Wei Zhang
human	WB	Pharmaceuticals (Basel); Use of Early Donated COVID-19 Convalescent Plasma Is Optimal to Preserve the Integrity of Lymphatic Endothelial Cells.; Authors - Nada Amri
human	WB	Mol Cell Endocrinol; Long noncoding RNA GAS5 accelerates diabetic wound healing and promotes lymphangiogenesis via miR-217/Prox1 axis.; Authors - Zhi-You He
human	WB	Oral Dis; Linagliptin's impact on lymphatic barrier and lymphangiogenesis in oral cancer with high glucose; Authors - Hongyu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (12-15-2023)

We used the concentration of 1:500 to get a very strong band. It is a big volume set.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lung cancer cell lines, lung cancer tissues

VEGFR3/FLT4 Antibody Western Blot validation (1:500 dilution) in Lung cancer cell lines, lung cancer tissues (Cat no:20712-1-AP)