Tested Applications
| Positive WB detected in | mouse spleen tissue, PC-3 cells, HeLa cells, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20712-1-AP targets FLT4/VEGFR3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | fms-related tyrosine kinase 4 |
| Calculated Molecular Weight | 153 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | NM_002020 |
| Gene Symbol | FLT4 |
| Gene ID (NCBI) | 2324 |
| RRID | AB_10733474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FLT4, also named VEGFR-3, belongs to the protein kinase superfamily, Tyr protein kinase family, and CSF-1/PDGF receptor subfamily. It is a receptor for VEGFC. FLT4 has a tyrosine-protein kinase activity. Defects in FLT4 cause lymphedema hereditary type 1 (LYH1A). Defects in FLT4 are found in juvenile hemangioma. VEGFR-3 is a highly glycosylated protein and migrates as bands with different molecular weights: an ~175 kDa precursor, an ~195 kDa mature form, an ~140 kDa non-glycosylated backbone, and a form that appears to be partially cleaved proteolytically in the extracellular domain to produce an ~125 kDa band(PMID: 15389531).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FLT4/VEGFR3 antibody 20712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FLT4/VEGFR3 Polyclonal antibody (Cat# 20712-1-AP) has 21 citations. These appear in journals including Molecular Cancer, Advanced Science, International Journal of Biological Sciences, Phytomedicine, Acta Pharmacologica Sinica, Stem Cell Research & Therapy, Journal of Ethnopharmacology, Scientific Reports, Cell Signaling, ACS Pharmacology & Translational Science, and Oncology Reports. Research fields span oncology (lung, thyroid, pancreatic, and hepatocellular cancers), lymphangiogenesis, angiogenesis, obesity, cardiac and renal fibrosis, spinal cord injury, and lymphatic endothelial biology.
| Species | Application | Title |
|---|---|---|
Mol Cancer m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma | ||
Adv Sci (Weinh) Single-Cell RNA Sequencing Reveals the Heterogeneity in Differentiation Trajectory and Tumor Microenvironment Leading to More Aggressive Phenotypes of Papillary Thyroid Cancer in Children and Young Adult Patients | ||
Int J Biol Sci PROTAC-Surufatinib Suppresses Pancreatic Neuroendocrine Neoplasms Progression by Inducing Ferroptosis through Inhibiting WNT/β-catenin Pathway Mediated by HMOX1 | ||
Phytomedicine Berberine promotes lacteal junction zippering and ameliorates diet-induced obesity through the RhoA/ROCK signaling pathway | ||
Acta Pharmacol Sin DsbA-L ameliorates renal aging and renal fibrosis by maintaining mitochondrial homeostasis | ||
Int J Biol Macromol Structural characterisation of a polysaccharide from Sanghuangporus vaninii and its synergistic anti-hepatocellular carcinoma activity in combination with sorafenib. |
Reviews
What customers say
The single review for this product reports a 4-star rating from a Western Blot application. The user tested it at a 1:500 dilution on lung cancer cell lines and lung cancer tissues, noting that it produced a very strong band. They also mentioned that the product comes in a large volume, which they found to be a positive aspect. Overall, the feedback is favorable, highlighting strong signal performance and generous quantity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shinford (Verified Customer) (12-15-2023) | We used the concentration of 1:500 to get a very strong band. It is a big volume set.
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