FMR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FMR1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 13755-1-AP

Synonyms

FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1

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Validation Data Gallery

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  WB analysis of Jurkat cells using 13755-1-AP

  WB result of FMR1 antibody (13755-1-AP, 1:1500) with si-Control and si-FMR1 transfected Jurkat cells. FMR1 gene has many isoforms with MW 59-72 kDa.

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  WB analysis of K-562 using 13755-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13755-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13755-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13755-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13755-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13755-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13755-1-AP

  IP Result of anti-FMRP (IP:13755-1-AP, 4ug; Detection:13755-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IP experiment of HeLa cells using 13755-1-AP

  IP result of anti-FMRP (13755-1-AP for IP and Detection).

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  IHC staining of human gliomas using 13755-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13755-1-AP (FMRP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 13755-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13755-1-AP (FMRP antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 13755-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13755-1-AP

  Immunofluorescent analysis of HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HeLa using 13755-1-AP

  Immunofluorescent analysis of Hela cells, using FMR1 antibody 13755-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

13755-1-AP targets FMR1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMR1 fusion protein Ag4697
• Full Name: fragile X mental retardation 1
• Calculated molecular weight: 632 aa, 71 kDa
• Observed molecular weight: 59-72 kDa
• GenBank accession number: BC038998
• Gene symbol: FMR1
• Gene ID (NCBI): 2332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Publications

Species	Application	Title
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	WB	Cancer Cell Int; MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.; Authors - Yu Men
human		Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marta (Verified Customer) (02-24-2020)

not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Brain mouse and rat

FMR1 Antibody Western Blot, validation (1/1000 dilution) in Brain mouse and rat (Cat no:13755-1-AP)