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c-FOS Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 26192-1-AP

Featured Product KD/KO validated

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Con: 37 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

c-FOS Antibody IHC mouse brain tissue 26192-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 26192-1-AP( c-FOS antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

c-FOS Antibody IF mouse brain tissue 26192-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 26192-1-AP (c-FOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

c-FOS Antibody WB HepG2 cells 26192-1-AP

WB result of c-FOS antibody (26192-1-AP, 1:1000) with si-Control and si-c-FOS transfected HepG2 cells.

c-FOS Antibody WB mouse embryo tissue 26192-1-AP

mouse embryo tissue were subjected to SDS PAGE followed by western blot with 26192-1-AP( c-FOS antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse embryo tissue, rat liver tissue, rat kidney tissue, HepG2 cells
Positive IHC detected in:
mouse brain tissue
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:200-1:800
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
c-FOS fusion protein Ag24340
Full name:
v-fos FBJ murine osteosarcoma viral oncogene homolog
Calculated molecular weight:
41 kDa
Observed molecular weight:
65 kDa
GenBank accession number:
BC004490
Gene ID (NCBI):
2353
Gene symbol
FOS
Synonyms
AP 1, C FOS, Cellular oncogene fos, c-FOS, FOS, G0S7, Proto oncogene c Fos

Background

c-FOS, also named as Proto-oncogene c-Fos and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. Upregulation of c-fos mRNA in a neuron indicates recent activity


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