c-FOS Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 26192-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 37 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse embryo tissue, rat liver tissue, rat kidney tissue, HepG2 cells

Positive IHC detected in:
mouse brain tissue

Positive IF detected in:
mouse brain tissue

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:200-1:800
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
v-fos FBJ murine osteosarcoma viral oncogene homolog

Calculated molecular weight:
41 kDa

Observed molecular weight:
65 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
FOS

Synonyms
AP 1, C FOS, Cellular oncogene fos, c-FOS, FOS, G0S7, Proto oncogene c Fos
Background

c-FOS, also named as Proto-oncogene c-Fos and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. FOS is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, FOS undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the FOS protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the FOS protein becomes nuclear. The calculated molecular weight of FOS is 40 kDa, but Phosphorylated FOS protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. Upregulation of c-fos mRNA in a neuron indicates recent activity


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