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FOXO3A Polyclonal antibody

FOXO3A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 10849-1-AP

Synonyms

AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, Neuro-2a cells, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10849-1-AP targets FOXO3A in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXO3A fusion protein Ag1289
Full Name forkhead box O3
Calculated Molecular Weight 673 aa, 71 kDa
Observed Molecular Weight 72-97 kDa
GenBank Accession NumberBC021224
Gene Symbol FOXO3
Gene ID (NCBI) 2309
RRIDAB_2247214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)

Protocols

Product Specific Protocols
WB protocol for FOXO3A antibody 10849-1-APDownload protocol
IHC protocol for FOXO3A antibody 10849-1-APDownload protocol
IP protocol for FOXO3A antibody 10849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis.

Authors - Shasha Yin
humanWB

Nat Commun

Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1.

Authors - Chen Xu
mouseWB

Aging Cell

Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling

Authors - Ruili Dang
mouse,humanWB,IF

Redox Biol

A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.

Authors - Qifeng Deng
WB

Environ Pollut

Response of lipid metabolism, energy supply, and cell fate in yellowstripe goby (Mugilogobius chulae) exposed to environmentally relevant concentrations atorvastatin

Authors - Yufei Zhao
mouse,ratWB

Oxid Med Cell Longev

Ketone Body β-Hydroxybutyrate Prevents Myocardial Oxidative Stress in Septic Cardiomyopathy.

Authors - Liwei Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Simona (Verified Customer) (03-03-2023)

The FOXO3A band is clearly visible in SH-SY5Y neuroblastoma cells, the last line, consisting of PC3 cells was considered as positive control but the signal was lower than SH-SY5Y. The product is useful.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y
FOXO3A Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y (Cat no:10849-1-AP)
FH

Valeria (Verified Customer) (11-11-2020)

Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human skeletal muscle sections (10um)
FOXO3A Antibody Immunofluorescence validation (1:50 dilution) in Human skeletal muscle sections (10um) (Cat no:10849-1-AP)
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain