• Featured Product
  • KD/KO Validated

FOXO3A Monoclonal antibody

FOXO3A Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, rat and More (1)







Cat no : 66428-1-Ig


AF6q21, AF6q21 protein, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66428-1-Ig targets FOXO3A in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, macaque
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FOXO3A fusion protein Ag1289
Full Name forkhead box O3
Calculated molecular weight 673 aa, 71 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC021224
Gene symbol FOXO3
Gene ID (NCBI) 2309
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa fulllength FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386)


Product Specific Protocols
WB protocol for FOXO3A antibody 66428-1-IgDownload protocol
IHC protocol for FOXO3A antibody 66428-1-IgDownload protocol
IF protocol for FOXO3A antibody 66428-1-IgDownload protocol
IP protocol for FOXO3A antibody 66428-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Neurosci

Role of FOXO3 Activated by HIV-1 Tat in HIV-Associated Neurocognitive Disorder Neuronal Apoptosis.

Authors - Huaqian Dong

Cell Death Discov

TGR5 deficiency aggravates hepatic ischemic/reperfusion injury via inhibiting SIRT3/FOXO3/HIF-1ɑ pathway.

Authors - Qi Wang

Free Radic Res

SIRT2 tyrosine nitration by peroxynitrite in response to renal ischemia/reperfusion injury.

Authors - Yan Wang


Protective effect of limonin against doxorubicin-induced cardiotoxicity via activating nuclear factor - like 2 and Sirtuin 2 signaling pathways.

Authors - Jie Deng

Int J Rheum Dis

Protective effects of 2,3,5,4-tetrahydroxystilbene-2-o-β-D-glucoside against osteoporosis: Current knowledge and proposed mechanisms.

Authors - Jinkang Zhang

Exp Cell Res

Alleviation of CCCP-induced mitochondrial injury by augmenter of liver regeneration via the PINK1/Parkin pathway-dependent mitophagy.

Authors - Jing Zhang