FOXO4 Polyclonal antibody

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  WB analysis of human heart using 19121-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19121-1-AP (FOXO4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 19121-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19121-1-AP (FOXO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 19121-1-AP

  Immunofluorescent analysis of HepG2 cells, using FOXO4 antibody 19121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

FOXO4 Polyclonal Antibody for WB, IF, ELISA

Cat No. 19121-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

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Tested Applications

• Positive WB detected in: human heart tissue, mouse heart tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

19121-1-AP targets FOXO4 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: forkhead box O4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: NM_005938
• Gene Symbol: FOXO4
• Gene ID (NCBI): 4303
• RRID: AB_2106957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P98177
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the ins signaling pathway. It binds to ins-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IF	Neurosci Lett; Expression of FOXO transcription factors in the brain following traumatic brain injury.; Authors - Xi-Lin Liu