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  • KD/KO Validated

Fascin Polyclonal antibody

Fascin Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14384-1-AP

Synonyms

55 kDa actin bundling protein, FAN1, Fascin, Fascin 1,Fascin, FSCN1, HSN, p55, Singed like protein, SNL



Tested Applications

Positive WB detected inHeLa cells, mouse thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14384-1-AP targets Fascin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fascin fusion protein Ag5816
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Calculated molecular weight 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000521
Gene symbol FSCN1
Gene ID (NCBI) 6624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.

Protocols

Product Specific Protocols
WB protocol for Fascin antibody 14384-1-APDownload protocol
IHC protocol for Fascin antibody 14384-1-APDownload protocol
IF protocol for Fascin antibody 14384-1-APDownload protocol
FC protocol for Fascin antibody 14384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis.

Authors - Mei-Ling Xu
mouseWB

Immunol Lett

Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.

Authors - Peiyu Gao
  • KD Validated
humanWB

J Exp Clin Cancer Res

The role of microRNA-133b and its target gene FSCN1 in gastric cancer.

Authors - Lihua Guo
humanWB

Exp Cell Res

Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.

Authors - Jones Lamptey
  • KD Validated
humanWB

Int J Biol Macromol

Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro.

Authors - Ajab Khan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval NaCit 0.1M ph6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Retina/Brain
Fascin Antibody Immunohistochemistry, validation (1:100 dilution) in Retina/Brain (Cat no:14384-1-AP)