|Positive WB detected in||HeLa cells, mouse thymus tissue|
|Positive IHC detected in||human tonsillitis tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14384-1-AP targets Fascin in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fascin fusion protein Ag5816|
|Full Name||fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC000521|
|Gene ID (NCBI)||6624|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.
|Product Specific Protocols|
|WB protocol for Fascin antibody 14384-1-AP||Download protocol|
|IHC protocol for Fascin antibody 14384-1-AP||Download protocol|
|IF protocol for Fascin antibody 14384-1-AP||Download protocol|
|FC protocol for Fascin antibody 14384-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Yes-associated protein upregulates filopodia formation to promote alveolar epithelial-cell phagocytosis.
J Exp Clin Cancer Res
The role of microRNA-133b and its target gene FSCN1 in gastric cancer.
Exp Cell Res
Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.
Int J Biol Macromol
Recombinant porcine NK-lysin inhibits the invasion of hepatocellular carcinoma cells in vitro.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (01-24-2018)
Antigen retrieval NaCit 0.1M ph6