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  • KD/KO Validated

FUS/TLS Monoclonal antibody

FUS/TLS Monoclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3A10B5

Cat no : 60160-1-Ig

Synonyms

75 kDa DNA pairing protein, FUS, FUS CHOP, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human brain (FTLD) tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman brain(ALS) tissue
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60160-1-Ig targets FUS/TLS in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, Drosophila
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FUS/TLS fusion protein Ag2150
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC026062
Gene symbol FUS
Gene ID (NCBI) 2521
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.

Protocols

Product Specific Protocols
WB protocol for FUS/TLS antibody 60160-1-IgDownload protocol
IHC protocol for FUS/TLS antibody 60160-1-IgDownload protocol
IP protocol for FUS/TLS antibody 60160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

J Cell Biol

The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.

Authors - Yasuda Kyota K
  • KD Validated

Brain

Motor neuron degeneration in spastic paraplegia 11 mimics amyotrophic lateral sclerosis lesions.

Authors - Paola S Denora
humanIHC

Acta Neuropathol

Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.

Authors - Neumann Manuela M
humanWB,IF

Ann Neurol

A Novel Missense Mutation of CMT2P Alters Transcription Machinery.

Authors - Bo Hu
humanWB,RIP

Cancer Lett

A novel promoter-associated non-coding small RNA paGLI1 recruits FUS/P65 to transactivate GLI1 gene expression and promotes infiltrating glioma progression.

Authors - Jinjing Zhong
  • KD Validated
humanWB

PLoS Genet

FUS Interacts with HSP60 to Promote Mitochondrial Damage.

Authors - Jianwen Deng