FXN Polyclonal antibody

• Featured Product
• KD/KO Validated

FXN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (3)

Cat no : 14147-1-AP

Synonyms

FA, Extramitochondrial frataxin, EC:1.16.3.1, dFXN, d FXN

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Validation Data Gallery

• 

  WB analysis using 14147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14147-1-AP (FXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14147-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14147-1-AP (FXN antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, Jurkat cells, K-562 cells, Raji cells, mouse liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

14147-1-AP targets FXN in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FXN fusion protein Ag5316
• Full Name: frataxin
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC048097
• Gene Symbol: FXN
• Gene ID (NCBI): 2395
• RRID: AB_2231876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXN, also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574)

Publications

Species	Application	Title
human	WB	Blood; Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.; Authors - Floriane Petit
human	WB	Brain; Autosomal recessive cerebellar ataxia caused by a homozygous mutation in PMPCA.; Authors - Karine Choquet
mouse	WB	Neurobiol Dis; Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin; Authors - Daniel Fil
mouse	WB	J Nutr Biochem; Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction; Authors - Jingjing Liu
human	WB,IHC	Sci Rep; Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich's ataxia.; Authors - Joseph F Nabhan
human	WB	Hum Mol Genet; Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia.; Authors - Yanjie Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mariana (Verified Customer) (01-06-2021)

Failed to detect endogenous FXN in IPSC derived Cardiomyocytes from Friedreich Ataxia patients and controls.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: IPSC cardiomyocytes

FH

Zhuqing (Verified Customer) (11-26-2020)

In mouse cells, it can detect mature frataxin.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 mouse myoblast

FH

Daniel (Verified Customer) (08-20-2019)

Works fine for WB in mouse brain and heart tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain