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FXN Polyclonal antibody

FXN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14147-1-AP


CyaY, d FXN, FA, FARR, frataxin, Frataxin(81 210), Frataxin, mitochondrial, FRDA, Friedreich ataxia protein, FXN, i FXN Frataxin(56 210), m56 FXN Frataxin(78 210), m78 FXN Frataxin mature form, m81 FXN, X25

Tested Applications

Positive WB detected inHCT 116 cells, Jurkat cells, K-562 cells, Raji cells, mouse liver tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14147-1-AP targets FXN in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXN fusion protein Ag5316
Full Name frataxin
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC048097
Gene Symbol FXN
Gene ID (NCBI) 2395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXN, also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574)


Product Specific Protocols
WB protocol for FXN antibody 14147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.

Authors - Floriane Petit


Autosomal recessive cerebellar ataxia caused by a homozygous mutation in PMPCA.

Authors - Karine Choquet

Neurobiol Dis

Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin

Authors - Daniel Fil

J Nutr Biochem

Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction

Authors - Jingjing Liu

Sci Rep

Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich's ataxia.

Authors - Joseph F Nabhan

Hum Mol Genet

Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia.

Authors - Yanjie Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Mariana (Verified Customer) (01-06-2021)

Failed to detect endogenous FXN in IPSC derived Cardiomyocytes from Friedreich Ataxia patients and controls.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: IPSC cardiomyocytes

Zhuqing (Verified Customer) (11-26-2020)

In mouse cells, it can detect mature frataxin.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12 mouse myoblast

Daniel (Verified Customer) (08-20-2019)

Works fine for WB in mouse brain and heart tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain