FXN Polyclonal antibody

FXN Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14147-1-AP

Synonyms

CyaY, d FXN, FA, FARR, frataxin, Frataxin(81 210), Frataxin, mitochondrial, FRDA, Friedreich ataxia protein, FXN, i FXN Frataxin(56 210), m56 FXN Frataxin(78 210), m78 FXN Frataxin mature form, m81 FXN, X25



Tested Applications

Positive WB detected inMDA-MB-453s cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

14147-1-AP targets FXN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FXN fusion protein Ag5316
Full Name frataxin
Calculated molecular weight 23 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC048097
Gene symbol FXN
Gene ID (NCBI) 2395
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXN, also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574)

Protocols

Product Specific Protocols
WB protocol for FXN antibody 14147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochim Biophys Acta

Quantitative proteomics in Friedreich's ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis.

Authors - Lorène Télot
humanWB

Front Neurosci

Selected Histone Deacetylase Inhibitors Reverse the Frataxin Transcriptional Defect in a Novel Friedreich's Ataxia Induced Pluripotent Stem Cell-Derived Neuronal Reporter System.

Authors - Anna M Schreiber
mouseWB

Dis Model Mech

Mitochondrial damage and senescence phenotype of cells derived from a novel frataxin G127V point mutation mouse model of Friedreich's ataxia.

Authors - Daniel Fil
mouseWB

Toxicology

Oxidative stress-dependent frataxin inhibition mediated alcoholic hepatocytotoxicity through ferroptosis.

Authors - Jingjing Liu
  • KD Validated
ratWB

Phytomedicine

Salidroside inhibits doxorubicin-induced cardiomyopathy by modulating a ferroptosis-dependent pathway.

Authors - Hang Chen
humanWB

Hum Mol Genet

Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia.

Authors - Yanjie Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mariana (Verified Customer) (01-06-2021)

Failed to detect endogenous FXN in IPSC derived Cardiomyocytes from Friedreich Ataxia patients and controls.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: IPSC cardiomyocytes
FH

Zhuqing (Verified Customer) (11-26-2020)

In mouse cells, it can detect mature frataxin.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12 mouse myoblast
FH

Daniel (Verified Customer) (08-20-2019)

Works fine for WB in mouse brain and heart tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain