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Frataxin Polyclonal antibody

Frataxin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14147-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

FXN, CyaY, d FXN, dFXN, EC:1.16.3.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHCT 116 cells, Jurkat cells, K-562 cells, Raji cells, mouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14147-1-AP targets Frataxin in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Frataxin fusion protein Ag5316 Predict reactive species
Full Name frataxin
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 14-23 kDa
GenBank Accession NumberBC048097
Gene Symbol FXN
Gene ID (NCBI) 2395
RRIDAB_2231876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16595
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Frataxin (FXN), also named as FRDA, X25, m81-FXN, d-FXN, m78-FXN and i-FXN, belongs to the frataxin family. It promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe2+ to proteins involved in these pathways. FXN may play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe2+ to Fe3+; the oligomeric form but not the monomeric form has in vitro ferroxidase activity. FXN is cleaved to be 4 chains. FXN is expressed in the cytoplasm as a 210 amino acid (AA) precursor protein (pFXN; 23 KDa) that is translocated into mitochondria where it is processed by two consecutive steps into iFXN (FXN 42-210; 19 KDa) and finally mFXN (81-210; 14.2 KDa), which is functional. (PMID: 26704351, PMID: 26671574)

Protocols

Product Specific Protocols
WB protocol for Frataxin antibody 14147-1-APDownload protocol
IHC protocol for Frataxin antibody 14147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Blood

Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.

Authors - Floriane Petit
humanWB

Brain

Autosomal recessive cerebellar ataxia caused by a homozygous mutation in PMPCA.

Authors - Karine Choquet
mouseWB

Neurobiol Dis

Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin

Authors - Daniel Fil
mouseWB

J Nutr Biochem

Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction

Authors - Jingjing Liu
humanWB,IHC

Sci Rep

Intrathecal delivery of frataxin mRNA encapsulated in lipid nanoparticles to dorsal root ganglia as a potential therapeutic for Friedreich's ataxia.

Authors - Joseph F Nabhan
humanWB

Hum Mol Genet

Premature transcription termination at the expanded GAA repeats and aberrant alternative polyadenylation contributes to the Frataxin transcriptional deficit in Friedreich's ataxia.

Authors - Yanjie Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mariana (Verified Customer) (01-06-2021)

Failed to detect endogenous FXN in IPSC derived Cardiomyocytes from Friedreich Ataxia patients and controls.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: IPSC cardiomyocytes
FH

Zhuqing (Verified Customer) (11-26-2020)

In mouse cells, it can detect mature frataxin.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12 mouse myoblast
FH

Daniel (Verified Customer) (08-20-2019)

Works fine for WB in mouse brain and heart tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain
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