Phospholemman/FXYD1 Polyclonal antibody
Phospholemman/FXYD1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13721-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue, mouse kidney tissue, human brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 19 publications below |
| IF | See 1 publications below |
Product Information
13721-1-AP targets Phospholemman/FXYD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, rabbit, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospholemman/FXYD1 fusion protein Ag4669 |
| Full Name | FXYD domain containing ion transport regulator 1 |
| Calculated molecular weight | 92 aa, 10 kDa |
| Observed molecular weight | 10-15 kDa |
| GenBank accession number | BC032800 |
| Gene symbol | FXYD1 |
| Gene ID (NCBI) | 5348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospholemman/FXYD1 antibody 13721-1-AP | Download protocol |
| IHC protocol for Phospholemman/FXYD1 antibody 13721-1-AP | Download protocol |
| IP protocol for Phospholemman/FXYD1 antibody 13721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Appl Physiol (1985) Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men. | ||
Am J Physiol Cell Physiol Phospholemman is not required for the acute stimulation of Na,K-ATPase α2 activity during skeletal muscle fatigue. | ||
J Appl Physiol (1985) Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners. | ||
Physiol Rep Skeletal muscle proteins important for work capacity are altered with type 2 diabetes - Effect of 10-20-30 training. | ||
J Appl Physiol (1985) No additive effect of acetaminophen when co-ingested with caffeine on cycling performance in well-trained young men. | ||
Eur J Appl Physiol Muscle variables of importance for physiological performance in competitive football. |
