Phospholemman/FXYD1 Polyclonal antibody

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  WB analysis of human skeletal muscle using 13721-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13721-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13721-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13721-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13721-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13721-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 13721-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13721-1-AP (Phospholemman/FXYD1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 13721-1-AP

  IP result of anti-Phospholemman/FXYD1 (IP:13721-1-AP, 4ug; Detection:13721-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of mouse heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13721-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13721-1-AP (Phospholemman/FXYD1 antibody) at dilution of 1:50 (under 40x lens).

Phospholemman/FXYD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 13721-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

FXYD1, Phospholemman, Phospholemman/FXYD1, PLM

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue, mouse kidney tissue, human brain tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IF: See 1 publications below

Product Information

13721-1-AP targets Phospholemman/FXYD1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Phospholemman/FXYD1 fusion protein Ag4669
• Full Name: FXYD domain containing ion transport regulator 1
• Calculated Molecular Weight: 92 aa, 10 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC032800
• Gene Symbol: FXYD1
• Gene ID (NCBI): 5348
• RRID: AB_2108296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00168
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

Publications

Species	Application	Title
mouse	WB,IF	Sci Rep; Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.; Authors - Xiaomin Su
rat	WB	J Gen Physiol; Distinct α2 Na,K-ATPase membrane pools are differently involved in early skeletal muscle remodeling during disuse.; Authors - Violetta V Kravtsova
rat	WB	J Biol Chem; The nicotinic acetylcholine receptor and the Na,K-ATPase alpha2 isoform interact to regulate membrane electrogenesis in skeletal muscle.; Authors - Heiny Judith A JA
rat	WB	J Physiol; Fatigue-induced change in T-system excitability and its major cause in rat fast-twitch skeletal muscle in vivo.; Authors - Daiki Watanabe
human	WB	J Physiol; Cycling with blood flow restriction improves performance and muscle K+ regulation and alters the effect of anti-oxidant infusion in humans.; Authors - Danny Christiansen
human,mouse,rat	WB	Am J Physiol Endocrinol Metab; Effect of exercise and training on phospholemman phosphorylation in human skeletal muscle.; Authors - Benziane Boubacar B