Phospholemman/FXYD1 Polyclonal antibody

Phospholemman/FXYD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 13721-1-AP


FXYD1, Phospholemman, Phospholemman/FXYD1, PLM

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue, mouse kidney tissue, human brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13721-1-AP targets Phospholemman/FXYD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Phospholemman/FXYD1 fusion protein Ag4669
Full Name FXYD domain containing ion transport regulator 1
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC032800
Gene symbol FXYD1
Gene ID (NCBI) 5348
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)


Product Specific Protocols
WB protocol for Phospholemman/FXYD1 antibody 13721-1-APDownload protocol
IHC protocol for Phospholemman/FXYD1 antibody 13721-1-APDownload protocol
IP protocol for Phospholemman/FXYD1 antibody 13721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Appl Physiol (1985)

Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.

Authors - Morten Hostrup

Am J Physiol Cell Physiol

Phospholemman is not required for the acute stimulation of Na,K-ATPase α2 activity during skeletal muscle fatigue.

Authors - Palanikumar Manoharan

J Appl Physiol (1985)

Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.

Authors - Casper Skovgaard

Physiol Rep

Skeletal muscle proteins important for work capacity are altered with type 2 diabetes - Effect of 10-20-30 training.

Authors - Thomas Baasch-Skytte

J Appl Physiol (1985)

No additive effect of acetaminophen when co-ingested with caffeine on cycling performance in well-trained young men.

Authors - Søren Jessen

Eur J Appl Physiol

Muscle variables of importance for physiological performance in competitive football.

Authors - Magni Mohr