|Positive WB detected in||human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue, mouse kidney tissue, human brain tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1500|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13721-1-AP targets Phospholemman/FXYD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rabbit, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phospholemman/FXYD1 fusion protein Ag4669|
|Full Name||FXYD domain containing ion transport regulator 1|
|Calculated molecular weight||92 aa, 10 kDa|
|Observed molecular weight||10-15 kDa|
|GenBank accession number||BC032800|
|Gene ID (NCBI)||5348|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)
|Product Specific Protocols|
|WB protocol for Phospholemman/FXYD1 antibody 13721-1-AP||Download protocol|
|IHC protocol for Phospholemman/FXYD1 antibody 13721-1-AP||Download protocol|
|IP protocol for Phospholemman/FXYD1 antibody 13721-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Appl Physiol (1985)
Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.
Am J Physiol Cell Physiol
Phospholemman is not required for the acute stimulation of Na,K-ATPase α2 activity during skeletal muscle fatigue.
J Appl Physiol (1985)
Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.
Skeletal muscle proteins important for work capacity are altered with type 2 diabetes - Effect of 10-20-30 training.
Eur J Appl Physiol
Muscle variables of importance for physiological performance in competitive football.
Scand J Med Sci Sports
The effect of repeated periods of speed endurance training on performance, running economy and muscle adaptations (113/120 characters).