"DYKDDDDK tag Antibodies" Comparison
View side-by-side comparison of DYKDDDDK tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66008-4-Ig targets DYKDDDDK tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, IP-MS, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, yeast, recombinant protein |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | DYKDDDDK tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | FLAGTAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| IF protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| IP protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| WB protocol for DYKDDDDK tag antibody 66008-4-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DYKDDDDK tag Monoclonal antibody (8H6A10), catalog number 66008-4-Ig, has accumulated 1,162 citations across top-tier journals including Nature, Science, Cell, Immunity, Nat Commun, Nat Cell Biol, Autophagy, Redox Biol, J Clin Invest, and Theranostics. Associated research fields span oncology, immunology, metabolism, neurology, virology, cardiology, autophagy, epigenetics, RNA modification, and structural biology, reflecting its broad utility as a versatile tag detection reagent.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Signal Transduct Target Ther Low-input redoxomics facilitates global identification of metabolic regulators of oxidative stress in the gut | ||
Signal Transduct Target Ther A20 promotes colorectal cancer immune evasion by upregulating STC1 expression to block "eat-me" signal | ||
Signal Transduct Target Ther KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma | ||
Signal Transduct Target Ther APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients. | ||
Cancer Cell Overcoming tyrosine kinase inhibitor resistance in lung cancer brain metastasis with CTLA4 blockade |
Reviews
What customers say
Users generally rate this antibody positively, with most giving 5 stars. Reviewers highlight its high specificity, low background signal, and reliable performance in Western blot and immunofluorescence. The manufacturer-recommended dilutions were reported to work well, with staining patterns consistent with expected target localization. One reviewer noted it performed adequately for IP but preferred Sigma's anti-Flag M2 antibody for that application. Overall, the antibody is well-regarded for detection-based assays like WB and IF, with minor reservations for IP use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (07-21-2026) | An excellent antibody, suitable for both IF and WB. No background signal. Highly specific.
|
Meixin (Verified Customer) (10-14-2025) | In my hands, the antibody demonstrated high specificity and good signal-to-noise ratio in both Western blotting and immunohistochemistry. The dilution ratio recommended by the manufacturer worked well, and the staining pattern was consistent with the expected target localization.
|
Arezousadat (Verified Customer) (04-22-2025) |
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Boyan (Verified Customer) (12-12-2022) | worked very well
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Chun (Verified Customer) (06-19-2022) | This antibody
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Céline (Verified Customer) (03-29-2022) | I have compared with anti-Flag M2 from Sigma in an IP experiment and I found the M2 antibody better.
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