DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for IP, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1C1D2

Cat no : 60002-1-Ig

Synonyms


"DDDDK tag Antibodies" Comparison

View side-by-side comparison of DDDDK tag antibodies from other vendors to find the one that best suits your research needs.
  • IP experiment of Recombinant protein using 60002-1-Ig

    IP experiment of Recombinant protein using 60002-1-Ig

    IP Result of anti-DDDDK tag (IP:60002-1-Ig, 3ug; Detection:20543-1-AP 1:2000) with Recombinant protein protein lysate 1280ug.


Tested Applications

Positive WB detected inRecombinant Protein
Positive IP detected inRecombinant protein protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Sample-dependent, check data in validation data gallery

Product Information

60002-1-Ig targets DDDDK tag in WB, IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

Protocols

Product Specific Protocols
IP protocol for DDDDK tag antibody 60002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Aging (Albany NY)

HOXB9 enhances the ability of lung cancer cells to penetrate the blood-brain barrier.

Authors - HongShan Zheng
WB

Cell Death Dis

TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.

Authors - Sha Zhou
WB,IP

Curr Microbiol

Host filamentous actin is associated with Heliothis armigera single nucleopolyhedrosis virus (HaSNPV) nucleocapsid transport to the host nucleus.

Authors - Xu H H
humanWB

Biol Open

ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.

Authors - Sayaka Fujisawa
WB

Mol Cell Biochem

INMAP, a novel truncated version of POLR3B, represses AP-1 and p53 transcriptional activity.

Authors - Yunlei Zhou Z
WB,IP

Mol Cell Biochem

RTN3 inducing apoptosis is modulated by an adhesion protein CRELD1.

Authors - Xiang Rong R