DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for IP, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IP,ELISA

Conjugate

Unconjugated

CloneNo.

1C1D2

Cat no : 60002-1-Ig

Synonyms



Tested Applications

Positive WB detected inRecombinant Protein
Positive IP detected inRecombinant protein protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Sample-dependent, check data in validation data gallery

Product Information

60002-1-Ig targets DDDDK tag in WB, IP,ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name DDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

Protocols

Product Specific Protocols
IP protocol for DDDDK tag antibody 60002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IP

Curr Microbiol

Host filamentous actin is associated with Heliothis armigera single nucleopolyhedrosis virus (HaSNPV) nucleocapsid transport to the host nucleus.

Authors - Xu H H
WB

Mol Cell Biochem

INMAP, a novel truncated version of POLR3B, represses AP-1 and p53 transcriptional activity.

Authors - Yunlei Zhou Z
WB,IP

Mol Cell Biochem

RTN3 inducing apoptosis is modulated by an adhesion protein CRELD1.

Authors - Xiang Rong R
WB

Mol Biol Rep

Overexpression of APRIN inhibits differentiation and proliferation and promotes apoptosis in P19 embryonal carcinoma cells.

Authors - Zhou Xiang X
WB,IP

Apoptosis

Adaptor FADD is recruited by RTN3/HAP in ER-bound signaling complexes.

Authors - Xiang Rong R
WB

J Virol

Enterovirus D68 Protease 2Apro Targets TRAF3 to Subvert Host Innate Immune Responses.

Authors - Jun Kang