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G-CSF Polyclonal antibody

G-CSF Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 17185-1-AP


C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MOLT-4 cells, MCF-7 cells, K-562 cells
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17185-1-AP targets G-CSF in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen G-CSF fusion protein Ag10968
Full Name colony stimulating factor 3 (granulocyte)
Calculated Molecular Weight 200 aa, 22 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC033245
Gene Symbol CSF3
Gene ID (NCBI) 1440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.


Product Specific Protocols
WB protocol for G-CSF antibody 17185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Immunol

Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis.

Authors - Zheng Zhang

Int J Mol Sci

A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer

Authors - Wei Jiang
  • KD Validated