G-CSF Polyclonal antibody

G-CSF Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17185-1-AP

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

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Validation Data Gallery

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  IHC staining of human pancreas using 17185-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17185-1-AP (G-CSF Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 17185-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17185-1-AP (G-CSF Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 17185-1-AP

  Immunofluorescent analysis of HeLa cells using 17185-1-AP (G-CSF antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 17185-1-AP is G-CSF Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: G-CSF fusion protein Ag10968
• Full Name: colony stimulating factor 3 (granulocyte)
• Calculated molecular weight: 200 aa, 22 kDa
• GenBank accession number: BC033245
• Gene symbol: CSF3
• Gene ID (NCBI): 1440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

Publications

Species	Application	Title
human	IHC	Mol Immunol; Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis.; Authors - Zheng Zhang