G-CSF Polyclonal antibody
G-CSF Polyclonal Antibody for IF, IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 17185-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
Product Information
The immunogen of 17185-1-AP is G-CSF Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | G-CSF fusion protein Ag10968 |
Full Name | colony stimulating factor 3 (granulocyte) |
Calculated molecular weight | 200 aa, 22 kDa |
GenBank accession number | BC033245 |
Gene symbol | CSF3 |
Gene ID (NCBI) | 1440 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.
Protocols
Product Specific Protocols | |
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IHC protocol for G-CSF antibody 17185-1-AP | Download protocol |
IF protocol for G-CSF antibody 17185-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Immunol Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis. |