GA repeat Polyclonal antibody

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  ELISA experiment of peptide using 24492-1-AP

  GA repeat Antibody (24492-1-AP) tested in ELISA. GA repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of GA repeat antibody (24492-1-AP) . Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

GA repeat Polyclonal Antibody for ELISA

Cat No. 24492-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, Dot Blot, ELISA

Synonyms

C9RAN, GA repeat, GAGAGAGAGA, GGGCC, poly GA

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Tested Applications

• Positive ELISA detected in: peptide

Recommended dilution

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

24492-1-AP targets GA repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GA repeat
• RRID: AB_2879571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "GA repeat" sequence. This antibody detects the GAGAGAGAGA peptide with dilution 1:32,000 in Elisa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Neurosci; Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.; Authors - Kornélia Szebényi
mouse	IHC	EMBO Mol Med; Active poly-GA vaccination prevents microglia activation and motor deficits in a C9orf72 mouse model.; Authors - Qihui Zhou
human	WB, IHC	Neuropathol Appl Neurobiol; Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.; Authors - Yvonne Davidson
human	WB,IF	Hum Mol Genet; Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.; Authors - Janis Bennion Callister
human		Stem Cells; C9orf72 Hexanucleotide Expansions Are Associated with Altered Endoplasmic Reticulum Calcium Homeostasis and Stress Granule Formation in Induced Pluripotent Stem Cell-Derived Neurons from Patients with Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.; Authors - Ruxandra Dafinca
human	WB	J Biol Chem; eIF5 stimulates the CUG initiation of RAN translation of poly-GA dipeptide repeat protein (DPR) in C9orf72 FTLD/ALS; Authors - Shiho Gotoh