GA repeat Polyclonal antibody

GA repeat Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, Dot Blot,ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 24492-1-AP

Synonyms

C9RAN, GA repeat, GAGAGAGAGA, GGGCC, poly GA

---

Validation Data Gallery

• 

  ELISA experiment of peptide using 24492-1-AP

  GA repeat Antibody (24492-1-AP) tested in ELISA. GA repeat peptide was coated onto the microplates at 1.5 µg/well and then incubated with dilution series of GA repeat antibody (24492-1-AP) . Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

---

Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

24492-1-AP targets GA repeat in WB, IHC, IF, Dot Blot,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: Drosophila, human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GA repeat
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "GA repeat" sequence. This antibody detects the GAGAGAGAGA peptide with dilution 1:32,000 in Elisa.

Publications

Species	Application	Title
human	WB, IHC	Neuropathol Appl Neurobiol; Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins.; Authors - Yvonne Davidson
Drosophila	Dot Blot	Cell Rep; Altered MICOS Morphology and Mitochondrial Ion Homeostasis Contribute to Poly(GR) Toxicity Associated with C9-ALS/FTD.; Authors - Shuangxi Li
		Hum Mol Genet; Smcr8 deficiency disrupts axonal transport-dependent lysosomal function and promotes axonal swellings and gain of toxicity in C9ALS/FTD mouse models.; Authors - Chen Liang
human	WB	J Cell Sci; SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.; Authors - Mirjana Malnar
human		Stem Cells; C9orf72 Hexanucleotide Expansions Are Associated with Altered Endoplasmic Reticulum Calcium Homeostasis and Stress Granule Formation in Induced Pluripotent Stem Cell-Derived Neurons from Patients with Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.; Authors - Ruxandra Dafinca
human	WB,IF	Hum Mol Genet; Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.; Authors - Janis Bennion Callister

Related Products

C9orf72 Rabbit Polyclonal antibody

22637-1-AP

C9orf72 Mouse Monoclonal antibody

66140-1-Ig

GP repeat Rabbit Polyclonal antibody

24494-1-AP

GR repeat Rabbit Polyclonal antibody

23978-1-AP

TDP-43 (human specific) Mouse Monoclonal antibody

60019-2-Ig

TDP-43 Rabbit Polyclonal antibody

10782-2-AP