GAB2 Polyclonal antibody

GAB2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22549-1-AP

Synonyms

GAB2, GRB2 associated binder 2, KIAA0571, pp100



Tested Applications

Positive WB detected inMCF-7 cells, mouse spleen tissue
Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22549-1-AP is GAB2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAB2 fusion protein Ag18192
Full Name GRB2-associated binding protein 2
Calculated molecular weight 676 aa, 74 kDa
Observed molecular weight 70-74 kDa
GenBank accession numberBC131711
Gene symbol GAB2
Gene ID (NCBI) 9846
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GAB2 antibody 22549-1-APDownload protocol
IHC protocol for GAB2 antibody 22549-1-APDownload protocol
IF protocol for GAB2 antibody 22549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Transl Res

Silencing of lncRNA HOXA11-AS inhibits cell migration, invasion, proliferation, and promotes apoptosis in human glioma cells via upregulating microRNA-125a: in vitro and in vivo studies.

Authors - Jianxin Jiang
humanWB

Front Physiol

Enhanced Autophagy in GAB1-Deficient Vascular Endothelial Cells Is Responsible for Atherosclerosis Progression.

Authors - Xin Qian