Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue|
|Positive IF detected in||HepG2 cells, Starvation treated HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18723-1-AP targets GABARAP in WB, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, monkey|
|Host / Isotype||Rabbit / IgG|
|Full Name||GABA(A) receptor-associated protein|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14-18 kDa|
|GenBank accession number||BC106748|
|Gene ID (NCBI)||11337|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.
ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis
ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1
PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.
ATF4 links ER stress with reticulophagy in glioblastoma cells.
ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hala (Verified Customer) (05-09-2023)
works very good in milk 5%
Anita (Verified Customer) (12-22-2020)
HeLa cell protein extract (5 micrograms)