GABARAP Polyclonal antibody

• Featured Product
• KD/KO Validated

GABARAP Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 18723-1-AP

Synonyms

FLC3B, GABARAP, MM46

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Validation Data Gallery

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  WB analysis of human placenta using 18723-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 18723-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells and untreated HepG2 cells using GABARAP antibody (18723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, A549 cells, HeLa cells, human heart tissue, mouse brain tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue
• Positive IF detected in: HepG2 cells, Starvation treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• CoIP: See 2 publications below

Product Information

18723-1-AP targets GABARAP in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, monkey, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GABA(A) receptor-associated protein
• Calculated molecular weight: 14 kDa
• Observed molecular weight: 14 kDa
• GenBank accession number: BC106748
• Gene symbol: GABARAP
• Gene ID (NCBI): 11337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Publications

Species	Application	Title
human,monkey	CoIP,WB	Curr Biol; ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.; Authors - Qingzhou Chen ; KO Validated
mouse	CoIP	Cell Death Dis; Protrudin modulates seizure activity through GABAA receptor regulation.; Authors - Xi Lu
human	WB	Cells; The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles.; Authors - Julia L Sanwald
mouse	WB	Eur J Cell Biol; The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.; Authors - Dorthe Labonté
human	WB	Sci Rep; Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.; Authors - Alexandra Boeske
human	WB	Cell Commun Signal; Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components.; Authors - Paula Lindner