GABARAP Polyclonal antibody

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  WB analysis using 18723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 18723-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18723-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18723-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 18723-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18723-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18723-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18723-1-AP

  IP result of anti-GABARAP (IP:18723-1-AP, 4ug; Detection:18723-1-AP 1:2000) with HeLa cells lysate 1200 ug.

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  IF Staining of HepG2 using 18723-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells and untreated HepG2 cells using GABARAP antibody (18723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 18723-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells and untreated HepG2 cells using 18723-1-AP (GABARAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 18723-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GABARAP (18723-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

GABARAP Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18723-1-AP

Publications(25)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

MM46, HT004, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, FLC3B

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells, Starvation treated HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IF: See 5 publications below
• CoIP: See 5 publications below

Product Information

18723-1-AP targets GABARAP in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GABA(A) receptor-associated protein
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC106748
• Gene Symbol: GABARAP
• Gene ID (NCBI): 11337
• RRID: AB_10603365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Publications

Species	Application	Title
human	WB	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	WB	Autophagy; ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis; Authors - Jinjin Zhang ; KD Validated
human	WB	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie ; KO Validated
	IF	Nat Commun; PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.; Authors - Shifan Wang
human	WB	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
mouse	WB	Autophagy; ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.; Authors - Mads Skytte Rasmussen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hala (Verified Customer) (05-09-2023)

works very good in milk 5%

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

GABARAP Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:18723-1-AP)

FH

Hala (Verified Customer) (04-28-2023)

works very good with milk blocking

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

FH

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5 micrograms)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

GABARAP Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:18723-1-AP)