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GABARAP Polyclonal antibody
GABARAP Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 18723-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue |
| Positive IF detected in | HepG2 cells, Starvation treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| CoIP | See 2 publications below |
Product Information
18723-1-AP targets GABARAP in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, monkey, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | GABA(A) receptor-associated protein |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 14-18 kDa |
| GenBank accession number | BC106748 |
| Gene symbol | GABARAP |
| Gene ID (NCBI) | 11337 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GABARAP antibody 18723-1-AP | Download protocol |
| IF protocol for GABARAP antibody 18723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
| ||
Cells The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles. | ||
Eur J Cell Biol The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors. | ||
Sci Rep Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization. | ||
Cell Commun Signal Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anita (Verified Customer) (12-22-2020) | HeLa cell protein extract (5 micrograms)
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