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  • KD/KO Validated

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18723-1-AP

Synonyms

FLC3B, GABARAP, MM46



Tested Applications

Positive WB detected inHeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue
Positive IF detected inHepG2 cells, Starvation treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18723-1-AP targets GABARAP in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GABA(A) receptor-associated protein
Calculated molecular weight 14 kDa
Observed molecular weight 14-18 kDa
GenBank accession numberBC106748
Gene symbol GABARAP
Gene ID (NCBI) 11337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Protocols

Product Specific Protocols
WB protocol for GABARAP antibody 18723-1-APDownload protocol
IF protocol for GABARAP antibody 18723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyCoIP,WB

Curr Biol

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

Authors - Qingzhou Chen
  • KO Validated
mouseCoIP

Cell Death Dis

Protrudin modulates seizure activity through GABAA receptor regulation.

Authors - Xi Lu
humanWB

Cells

The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles.

Authors - Julia L Sanwald
mouseWB

Eur J Cell Biol

The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.

Authors - Dorthe Labonté
humanWB

Sci Rep

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.

Authors - Alexandra Boeske
humanWB

Cell Commun Signal

Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components.

Authors - Paula Lindner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5 micrograms)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells
GABARAP Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:18723-1-AP)