GABARAP Polyclonal antibody

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• GABARAP Polyclonal antibody (18723-1-AP)

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• KD/KO Validated

GABARAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18723-1-AP

Publications(38)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

FLC3B, GABA(A) receptor-associated protein, Gamma-aminobutyric acid receptor-associated protein, HT004, MM46

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  WB analysis using 18723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 18723-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 18723-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 18723-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 18723-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human heart using 18723-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 18723-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 18723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 18723-1-AP

  IP result of anti-GABARAP (IP:18723-1-AP, 4ug; Detection:18723-1-AP 1:2000) with HeLa cells lysate 1200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 18723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18723-1-AP (GABARAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 18723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18723-1-AP (GABARAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 18723-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells and untreated HepG2 cells using GABARAP antibody (18723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 18723-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells and untreated HepG2 cells using 18723-1-AP (GABARAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 18723-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GABARAP (18723-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Starvation treated HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18723-1-AP targets GABARAP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: GABA(A) receptor-associated protein
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-18 kDa
• GenBank Accession Number: BC106748
• Gene Symbol: GABARAP
• Gene ID (NCBI): 11337
• RRID: AB_10603365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.; Authors - Linyu Sun
human	WB	Autophagy; ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis; Authors - Jinjin Zhang ; KD Validated
human	WB	Autophagy; ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1; Authors - Weihong Xie ; KO Validated
	IF	Nat Commun; PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.; Authors - Shifan Wang
human	WB	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
mouse	WB	Autophagy; ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.; Authors - Mads Skytte Rasmussen

Reviews

3.6 out of 5

3 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Hala (Verified Customer) (05-09-2023)

works very good in milk 5%

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

GABARAP Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:18723-1-AP)

Hala (Verified Customer) (04-28-2023)

works very good with milk blocking

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HepG2

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5 micrograms)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

GABARAP Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:18723-1-AP)