|Positive WB detected in||HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue|
|Positive IF detected in||HepG2 cells, Starvation treated HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18723-1-AP targets GABARAP in WB, IF, CoIP,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||GABA(A) receptor-associated protein|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14-18 kDa|
|GenBank accession number||BC106748|
|Gene ID (NCBI)||11337|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
Cell Death Dis
Protrudin modulates seizure activity through GABAA receptor regulation.
The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles.
Eur J Cell Biol
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.
Cell Commun Signal
Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anita (Verified Customer) (12-22-2020)
HeLa cell protein extract (5 micrograms)