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GABARAPL2-Specific Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 18724-1-AP

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Con: 48 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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Images See all

GABARAPL2-Specific Antibody WB COLO 320 cells 18724-1-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 18724-1-AP(GABARAPL2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

GABARAPL2-Specific Antibody IHC human heart tissue 18724-1-AP

Immunohistochemical of paraffin-embedded human heart using 18724-1-AP(GABARAPL2-Specific antibody) at dilution of 1:50 (under 10x lens)

GABARAPL2-Specific Antibody IHC human heart tissue 18724-1-AP

Immunohistochemical of paraffin-embedded human heart using 18724-1-AP(GABARAPL2-Specific antibody) at dilution of 1:50 (under 40x lens)

GABARAPL2-Specific Antibody FC HepG2 cells 18724-1-AP

1X10^6 HepG2 cells were stained with 0.2ug GABARAPL2-Specific antibody (18724-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
Positive IHC detected in:
human heart tissue, human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
GABA(A) receptor-associated protein-like 2
Calculated molecular weight:
14 kDa
Observed molecular weight:
14 kDa
GenBank accession number:
BC005985
Gene ID (NCBI):
11345
Gene symbol
GABARAPL2
Synonyms
ATG8, FLC3A, GABARAPL2, GATE 16, GATE16, GEF 2, GEF2

Background

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.


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