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GABARAPL2-Specific Polyclonal antibody
GABARAPL2-Specific Polyclonal Antibody for FC, IHC, WB, ELISA
Cat no : 18724-1-AP
|Positive WB detected in||COLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells|
|Positive IHC detected in||human heart tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18724-1-AP targets GABARAPL2-Specific in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||GABA(A) receptor-associated protein-like 2|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC005985|
|Gene ID (NCBI)||11345|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.
|Product Specific Protocols|
|WB protocol for GABARAPL2-Specific antibody 18724-1-AP||Download protocol|
|IHC protocol for GABARAPL2-Specific antibody 18724-1-AP||Download protocol|
|FC protocol for GABARAPL2-Specific antibody 18724-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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