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GABARAPL2-Specific Polyclonal antibody

GABARAPL2-Specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 18724-1-AP

Synonyms

ATG8, FLC3A, GABARAPL2, GATE 16, GATE16, GEF 2, GEF2



Tested Applications

Positive WB detected inCOLO 320 cells, human brain tissue, mouse brain tissue, mouse spleen tissue, PC-3 cells
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18724-1-AP targets GABARAPL2-Specific in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GABA(A) receptor-associated protein-like 2
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005985
Gene symbol GABARAPL2
Gene ID (NCBI) 11345
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL2 (also known as Golgi-associated ATPase enhancer of 16 kDa; GATE-16) was originally characterized as a factor essential for intra-Golgi protein transport. Later GABARAPL2 along with LC3 and GABARAP, were identified as the homologs of yeast ATG8 which is a unique ubiquitin-like protein essential for autophagy. When induced GABARAPL2 is first digested by ATG4 family enzymes to become its mature form: GABARAPL2-I, later conjugated to PE lipid as lipidated form: GABARAPL2-II. The lipidated form migrates faster than its mature form on SDS-PAGE gel. GABARAPL2 is strongly expressed in the brain. This antibody is specific to GABARAPL2. It has no cross-reaction to other GABARAP protein.

Protocols

Product Specific Protocols
WB protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
IHC protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
FC protocol for GABARAPL2-Specific antibody 18724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Rep

Human cytomegalovirus hijacks the autophagic machinery and LC3 homologs in order to optimize cytoplasmic envelopment of mature infectious particles.

Authors - Clémence Taisne
humanWB

Sci Rep

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.

Authors - Alexandra Boeske
humanWB

Sci Rep

A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency.

Authors - Arun Kumar Tharkeshwar
mouseWB

Mol Cell Biol

Defective autophagy and mTORC1 signaling in myotubularin null mice.

Authors - Fetalvero Kristina M KM
humanWB, IHC

Neurobiol Dis

Alteration of autophagosomal proteins in the brain of multiple system atrophy.

Authors - Tanji Kunikazu K
ratWB

Autophagy

CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.

Authors - Hyun-Kyu An