GABRG2 Polyclonal antibody

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• GABRG2 Polyclonal antibody (14104-1-AP)

GABRG2 Polyclonal Antibody for WB, IF-P, IP, ELISA

Cat No. 14104-1-AP Copied!

Publications (27)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Gamma-aminobutyric acid receptor subunit gamma-2, GABAAR subunit gamma-2, GABA(A) receptor subunit gamma-2, GABA A receptor gamma 2, ECA2

• 

  WB analysis using 14104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14104-1-AP (GABRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 14104-1-AP

  IP result of anti-GABRG2 (IP:14104-1-AP, 4ug; Detection:14104-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse cerebellum using 14104-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using GABRG2 antibody (14104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14104-1-AP targets GABRG2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5237

  Product name: Recombinant human GABRG2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 42-274 aa of BC059389

  Sequence: SDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGY

• Full Name: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• Calculated Molecular Weight: 467 aa, 54 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC059389
• Gene Symbol: GABRG2
• Gene ID (NCBI): 2566
• RRID: AB_10693527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18507
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.

Publications

What published studies show

Anti-GABRA1 antibody (Cat# 14104-1-AP) has accumulated 27 citations across 26 journals, including Nature Genetics, Nature Communications, Cell Reports, Redox Biology, Anesthesiology, and Journal of Neuroscience. The associated research fields primarily encompass neuroscience (epilepsy, GABA receptor signaling, synaptic plasticity, Alzheimer's disease, stroke), anesthesiology, pharmacology, neuroimmunology, and oncology. Common applications include Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Automatically generated from the 27 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nat Genet; Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements; Authors - Nakul M Shah
mouse	IF	Nat Commun; Ectoparasites enhance survival by suppressing host exploration and limiting dispersal; Authors - Pengbo Liu
mouse	WB	Redox Biol; Activation of nuclear receptor pregnane-X-receptor protects against abdominal aortic aneurysm by inhibiting oxidative stress; Authors - Zhi Shen
human	WB	Genes Dis; The Traf2 and NcK interacting kinase inhibitor NCB-0846 suppresses seizure activity involving the decrease of GRIA1; Authors - Min Wang
mouse	WB	Cell Death Dis; Transgenic overexpression of furin increases epileptic susceptibility.; Authors - Yi Yang
mouse	WB	Phytomedicine; Parishin from Gastrodia elata ameliorates DSS induced colitis and anxiety-like behavior in mice by regulating intestinal barrier function and microviota-gut-brain axis.; Authors - Xiaoxue Liu