GAC-specific Polyclonal antibody
GAC-specific Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 19958-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
19958-1-AP targets GAC-specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | glutaminase |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | AF158555 |
| Gene symbol | GLS |
| Gene ID (NCBI) | 2744 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| IHC protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| IP protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway. | ||
Int J Mol Sci Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase. | ||
RNA CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function. | ||
AIDS Res Hum Retroviruses Evidence for altered glutamine metabolism in HIV-1 infected primary human CD4+ T cells. | ||
Tumour Biol Silencing of GLS and overexpression of GLS2 genes cooperate in decreasing the proliferation and viability of glioblastoma cells. | ||
PLoS One Glutaminase is essential for the growth of triple-negative breast cancer cells with a deregulated glutamine metabolism pathway and its suppression synergizes with mTOR inhibition. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniel (Verified Customer) (09-16-2019) | The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.
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