GAC-specific Polyclonal antibody

GAC-specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19958-1-AP

Synonyms

AAD20, GAC, GAC-specific, GLS, GLS1, glutaminase C, K glutaminase, KIAA0838, L glutamine amidohydrolase



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

19958-1-AP targets GAC-specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name glutaminase
Calculated molecular weight 65 kDa
Observed molecular weight 58 kDa
GenBank accession numberAF158555
Gene symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.

Protocols

Product Specific Protocols
WB protocol for GAC-specific antibody 19958-1-APDownload protocol
IHC protocol for GAC-specific antibody 19958-1-APDownload protocol
IP protocol for GAC-specific antibody 19958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanWB

RNA

CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.

Authors - Chioniso P Masamha
humanWB

AIDS Res Hum Retroviruses

Evidence for altered glutamine metabolism in HIV-1 infected primary human CD4+ T cells.

Authors - Andrea Hegedus
humanWB

Tumour Biol

Silencing of GLS and overexpression of GLS2 genes cooperate in decreasing the proliferation and viability of glioblastoma cells.

Authors - Szeliga Monika M
humanWB

PLoS One

Glutaminase is essential for the growth of triple-negative breast cancer cells with a deregulated glutamine metabolism pathway and its suppression synergizes with mTOR inhibition.

Authors - Michael Lampa
humanWB,IF

PLoS One

T Helper Cell Activation and Expansion Is Sensitive to Glutaminase Inhibition under Both Hypoxic and Normoxic Conditions.

Authors - Zeynep Sener

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HK2 cells sustained on glucose or galactose
GAC-specific Antibody Western Blot, validation (1:1000 dilution) in HK2 cells sustained on glucose or galactose (Cat no:19958-1-AP)