GAC-specific Polyclonal antibody

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  WB analysis of A549 using 19958-1-AP

  WB result of GAC-specific antibody (19958-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAC-specific transfected A549 cells.

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  WB analysis using 19958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of mouse kidney using 19958-1-AP

  IP result of anti-GAC-specific (IP:19958-1-AP, 3ug; Detection:19958-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 19958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GAC-specific antibody (19958-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

GAC-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19958-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

GLS, KGA, GLS1, GAM, GAC

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human heart tissue, human kidney tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

19958-1-AP targets GAC-specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutaminase
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: AF158555
• Gene Symbol: GLS
• Gene ID (NCBI): 2744
• RRID: AB_10640899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94925
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection.; Authors - Maria L Balmer
mouse	IF	Cell Stem Cell; Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism.; Authors - Pia Sommerkamp
human	WB,IP	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB	Blood; Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.; Authors - Nathalie Jacque
	WB	Elife; Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor; Authors - Carolyn M Jablonowski
human	WB,IHC	Cell Death Discov; O-GlcNAcylation of glutaminase isoform KGA inhibits ferroptosis through activation of glutaminolysis in hepatoblastoma; Authors - Sijia Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK2 cells sustained on glucose or galactose

GAC-specific Antibody Western Blot, validation (1:1000 dilution) in HK2 cells sustained on glucose or galactose (Cat no:19958-1-AP)