Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19958-1-AP targets GAC-specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glutaminase |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | AF158555 |
| Gene Symbol | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_10640899 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94925 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| IHC protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| IP protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| WB protocol for GAC-specific antibody 19958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
19958-1-AP is a Glutaminase (GLS1) affinity-purified antibody with 27 total citations. Citations appear in journals including Cell Metabolism, Nature Structural & Molecular Biology, Blood, Cell Stem Cell, Cancer Communications, EBioMedicine, Oncogene, Oncotarget, International Journal of Molecular Sciences, and eLife. Research fields span glutamine metabolism, cancer (hepatocellular, breast, gastric, colorectal, glioblastoma), ferroptosis, immune cell biology, cardiac remodeling, mitochondrial function, and alternative splicing.
| Species | Application | Title |
|---|---|---|
Cell Metab Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection. | ||
Cancer Commun (Lond) WTAP-Mediated Glutaminase Splicing Bias Suppresses Ferroptosis in Hepatocellular Carcinoma. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Blood Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. | ||
Cell Stem Cell Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism. | ||
EBioMedicine Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway. |
Reviews
What customers say
One customer rated this product 4 out of 5 stars. The reviewer used it for Western Blot at a 1:1000 dilution on HK2 cells sustained on glucose or galactose. They noted that the nitrocellulose membrane was blocked with PBS containing 5% BSA and Tween-20. Overall, the review reflects a positive experience with the antibody's performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (09-16-2019) | The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.
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