|Positive WB detected in||mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:3000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
20746-1-AP targets GAD65 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||NM_000818|
|Gene ID (NCBI)||2572|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.
Clin Sci (Lond)
Prenatal cold exposure causes hypertension in offspring by hyperactivity of the sympathetic nervous system.
Aging (Albany NY)
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Spinal GABAergic neurons are under feed-forward inhibitory control driven by Aδ and C fibers in Gad2 td-Tomato mice.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lana (Verified Customer) (02-06-2020)
SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.