Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20746-1-AP targets GAD65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | NM_000818 |
| Gene Symbol | GAD65 |
| Gene ID (NCBI) | 2572 |
| RRID | AB_10949196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05329 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GAD65 antibody 20746-1-AP | Download protocol |
| WB protocol for GAD65 antibody 20746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GAD65 Polyclonal antibody (Cat# 20746-1-AP) has 29 citations spanning journals including Nature Cell Biology, Nature Communications, Journal of Experimental & Clinical Cancer Research, Advanced Science, Translational Psychiatry, Frontiers in Pharmacology, and BMC Biology. Research fields cover neuroscience (GABAergic signaling, depression, pain, visual plasticity), oncology (tumour growth, brain metastasis), metabolism (obesity, diet), immunology (autoimmune tolerance), and cardiovascular biology (hypertension).
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. | ||
Nat Commun High-fat diet disrupts a septal control on feeding to promote obesity in male mice. | ||
J Exp Clin Cancer Res GABA regulates metabolic reprogramming to mediate the development of brain metastasis in non-small cell lung cancer | ||
Adv Sci (Weinh) Gold Nanostrip Array-Mediated Wireless Electrical Stimulation for Accelerating Functional Neuronal Differentiation. | ||
Research (Wash D C) The Hypothalamic Medial Preoptic Area-Paraventricular Nucleus Circuit Modulates Depressive-Like Behaviors in a Mouse Model of Postpartum Depression | ||
Transl Psychiatry A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota. |
Reviews
What customers say
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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (08-06-2024) | GAD2/GAD65 (green) is supposed to mark cytoplasmatically or puncta-like my inhibitory neurons, but this lot did not seem to work, as it seems only artifacts marking around the nuclei.
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Lana (Verified Customer) (02-06-2020) | SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.
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