GAD65 Polyclonal antibody

GAD65 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (2)

Cat no : 20746-1-AP

Synonyms

GAD2, GAD-65, GAD 65, EC:4.1.1.15

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Validation Data Gallery

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  WB analysis using 20746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20746-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20746-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20746-1-AP

  IP result of anti-GAD65 (IP:20746-1-AP, 4ug; Detection:20746-1-AP 1:1000) with mouse brain tissue lysate 3440ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

20746-1-AP targets GAD65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_000818
• Gene Symbol: GAD65
• Gene ID (NCBI): 2572
• RRID: AB_10949196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.

Publications

Species	Application	Title
human,mouse	WB	Nat Cell Biol; Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.; Authors - De Huang
mouse	WB	Neurobiol Stress; A sex-dependent delayed maturation of visual plasticity induced by adverse experiences in early childhood.; Authors - Yueqin Liu
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
rat	WB	Sci Rep; Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension.; Authors - Hsin-Hung Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (08-06-2024)

GAD2/GAD65 (green) is supposed to mark cytoplasmatically or puncta-like my inhibitory neurons, but this lot did not seem to work, as it seems only artifacts marking around the nuclei.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Brain Cortex

GAD65 Antibody Immunofluorescence validation (1:50 dilution) in Human Brain Cortex (Cat no:20746-1-AP)

FH

Lana (Verified Customer) (02-06-2020)

SDS-PAGE: 15 ug/ul RIPA lysate of whole striatal tissue. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C, 1:1000Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissues, human.

GAD65 Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human. (Cat no:20746-1-AP)