GALNT7 Polyclonal antibody

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  WB analysis of HeLa using 13962-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13962-1-AP (GALNT7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13962-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13962-1-AP (GALNT7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13962-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13962-1-AP (GALNT7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

GALNT7 Polyclonal Antibody for WB, ELISA

Cat No. 13962-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GALNAC T7, GalNAcT7, GALNT7, pp GaNTase 7

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13962-1-AP targets GALNT7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GALNT7 fusion protein Ag4917
• Full Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)
• Calculated Molecular Weight: 75.4 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC047468
• Gene Symbol: GALNT7
• Gene ID (NCBI): 51809
• RRID: AB_2109009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86SF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GALNT7 is a member of GALNT family that are glycotransferases involved in oligosaccharide biosynthesis. Aberrant glycosylation has been linked to tumor development and progression. Increased expression of GALNT7 has been observed in cervical cancer cells. (PMID: 22399294)

Publications

Species	Application	Title
human	WB	Am J Transl Res; Resveratrol enhances MUC2 synthesis via the ANRIL-miR-34a axis to mitigate IBD; Authors - Xiangjun Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maggie (Verified Customer) (11-03-2023)

3 hours incubation

• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Cell culture, Protein purification
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: TRAMP-C2 Cells

GALNT7 Antibody Immunohistochemistry,Immunofluorescence,Flow Cytometry,Cell culture,Protein purification validation (1:200 dilution) in TRAMP-C2 Cells (Cat no:13962-1-AP)