GATA-4 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB

Cat No : 39893,39894

Synonyms

GATA-4, zinc finger, transcription factor, GATA, gata3 gata 4, mesodermal development, heart, cardiac, atrial septal defect type 2, cardiac muscle, smooth muscle, sample, dbdabs

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Validation Data Gallery

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  GATA-4 antibody (pAb) tested by Western blot. Western Blot: Nuclear extract (20 ug) of F9 cells (retinoic acid treated mouse teratocarcinoma F9 stem cell line, Active Motif Catalog no. 36113) probed with GATA-4 antibody at a dilution of 1:2,000.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB: 1:500 - 1:2,000 dilution

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This GATA-4 antibody was raised against a peptide derived from the C-terminus of human GATA-4.
• Full Name: GATA-4 antibody (pAb)
• Synonyms: GATA-4, zinc finger, transcription factor, GATA, gata3 gata 4, mesodermal development, heart, cardiac, atrial septal defect type 2, cardiac muscle, smooth muscle, sample, dbdabs
• Molecular weight: 55 kDa
• GenBank accession number: NP_002043
• RRID: AB_2615046
• Purification Method: Affinity Purified
• Buffer: Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

GATA-4 is a zinc finger protein and part of the GATA family of sequence specific DNA binding transcription factors that recognize a consensus DNA sequence containing GATA. GATA-4 is involved in the development of several mesodermal and endodermal structures. GATA-4 mutant mice exhibit severe heart malformations. Defects in GATA-4 are the cause of atrial septal defect type 2. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. GATA-4 also plays a role in regulating gene expression in cardiac and smooth muscle cell types.