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  • KD/KO Validated

GATA1 Polyclonal antibody

GATA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 10917-2-AP

Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT



Tested Applications

Positive WB detected inHL-60 cells, K-562 cells, NIH/3T3 cells, Raji cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10917-2-AP targets GATA1 in WB, IP, IHC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATA1 fusion protein Ag1350
Full Name GATA binding protein 1 (globin transcription factor 1)
Calculated molecular weight 43 kDa
Observed molecular weight 50-55 kDa, 40-45 kDa
GenBank accession numberBC009797
Gene symbol GATA1
Gene ID (NCBI) 2623
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ).

Protocols

Product Specific Protocols
WB protocol for GATA1 antibody 10917-2-APDownload protocol
IHC protocol for GATA1 antibody 10917-2-APDownload protocol
IP protocol for GATA1 antibody 10917-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Cell Biochem

GATA1 promotes colorectal cancer cell proliferation, migration and invasion via activating AKT signaling pathway.

Authors - Junhui Yu
  • KD Validated
humanWB,IHC

Oncotarget

The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer.

Authors - Yanan Zhang
mouseIHC

Genes (Basel)

Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector.

Authors - Takehito Sugasawa
humanWB

Front Immunol

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Authors - Chunlin Wang
humanWB,IHC

Oncogene

microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.

Authors - R Su