GATA2 Polyclonal antibody

GATA2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP

Conjugate

Unconjugated

Cat no : 11103-1-AP

Synonyms

GATA binding protein 2, GATA2, NFE1B



Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, K-562 cells, HepG2 cells, rat heart tissue, mouse liver tissue, HEK-293 cells, rat liver tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11103-1-AP targets GATA2 in WB, IHC, IF, ChIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GATA2 fusion protein Ag1557
Full Name GATA binding protein 2
Observed molecular weight50-60 kDa
GenBank accession numberBC018988
Gene symbol GATA2
Gene ID (NCBI) 2624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the molecular weight of GATA2? Is GATA2 post-translationally modified?

The molecular weight of GATA2 is 51 kDa. There are two known isoforms of GATA2 that use alternative first exons (PMID: 10731672). GATA2 can be phosphorylated, which regulates its activity (PMID: 7876160).

 What is the subcellular localization of GATA2?

GATA2 acts as a transcription factor in the nucleus. However, it can also be found in the cytoplasm (PMID: 25163535) and its phosphorylation promotes nuclear localization (PMID: 15837948).

 What is the tissue expression pattern of GATA2?

GATA2 was initially identified as a hematopoietic transcription factor regulating the differentiation of hematopoietic stem cells but is also present in early erythroid cells, mast cells, and megakaryocytes. Additionally, it is expressed in the central nervous system (PMID: 10357893).



Protocols

Product Specific Protocols
WB protocol for GATA2 antibody 11103-1-APDownload protocol
IHC protocol for GATA2 antibody 11103-1-APDownload protocol
IF protocol for GATA2 antibody 11103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases.

Authors - Manabu Ogoyama
humanWB

Mol Genet Genomics

GATA2 regulates the CAD susceptibility gene ADTRP rs6903956 through preferential interaction with the G allele.

Authors - Chunyan Luo
  • KD Validated
humanChIP,IF

Endocrinology

PGE2 and Thrombin Induce Myofibroblast Transdifferentiation via Activin A and CTGF in Endometrial Stromal Cells.

Authors - Kazuya Kusama
humanWB

PLoS Genet

The PU.1-Modulated MicroRNA-22 Is a Regulator of Monocyte/Macrophage Differentiation and Acute Myeloid Leukemia.

Authors - Chao Shen
humanIF

EMBO J

Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.

Authors - Yanni Ma