GBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GBP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

15

Cat no : 15303-1-AP

Synonyms

GBP 1, GBP1, GTP binding protein 1, HuGBP 1

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Validation Data Gallery

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  WB analysis using 15303-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 15303-1-AP (GBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 15303-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15303-1-AP (GBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15303-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15303-1-AP (GBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 15303-1-AP

  IP Result of anti-GBP1 (IP:15303-1-AP, 4ug; Detection:15303-1-AP 1:600) with human placenta tissue lysate 4000ug.

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  IHC staining of human spleen using 15303-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15303-1-AP (GBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 15303-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15303-1-AP (GBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 15303-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GBP1 antibody 15303-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human placenta tissue, human brain tissue, HUVEC cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

15303-1-AP targets GBP1 in WB, IP, IHC, IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP1 fusion protein Ag7562
• Full Name: GTP binding protein 1
• Calculated molecular weight: 68 kDa
• Observed molecular weight: 67 kDa
• GenBank accession number: BC002666
• Gene symbol: GBP1
• Gene ID (NCBI): 2633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP.

 What is the molecular weight of GBP1?

The molecular weight of GBP1 is 68kDa.

 What is the cellular localization of GBP1?

GBP1 is predominantly cytosolic, but under certain conditions is farnesylated and associated with intracellular membranes such as the Golgi (PMID: 15937107).

 What is the role of GBP1 in immunity?

GBP1 is induced in response to type I and type II IFN and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica (PMID: 21142871). These anti-pathogenic functions involve the recruitment to vacuolar compartments for the destruction of the pathogen, the assembly of innate immune complexes such as the inflammasome, or the initiation of autophagy.

 What is the role of GBP-1 in colorectal carcinoma?

In colorectal carcinoma (CRC), the presence of tumor-infiltrating T helper type 1 (Th1) cells participates in anti-tumoral responses and an improved clinical outcome (PMID: 17008531, 20927778). IFN promote the Th1 immune response, as well as GBP-1 expression. In CRC, GBP-1 expression acts as a tumor suppressor, where inhibition of tumor growth by GBP-1 is the result of its strong antiangiogenic activity as well as its direct anti-tumorigenic effect on tumor cells and is correlated with a better outcome (PMID: 23042300).

Publications

Species	Application	Title
human	WB,IHC	Aging (Albany NY); Immune profiling reveals prognostic genes in high-grade serous ovarian cancer.; Authors - Yong Wu ; KD Validated
human	WB	Arch Virol; Myxovirus resistance protein A inhibits hepatitis C virus replication through JAK-STAT pathway activation.; Authors - Hailong Wang
human	WB	PLoS One; In vivo interferon-gamma induced changes in gene expression dramatically alter neutrophil phenotype.; Authors - Daniel R Ambruso
mouse	WB,IF	Infect Genet Evol; Knockdown of GBP1 inhibits BCG-induced apoptosis in macrophage RAW 264.7 cells via p38/JNK pathway.; Authors - Jianhong Wang ; KD Validated
human	IF	Cell Microbiol; Toxoplasma gondii GRA60 is an effector protein that modulates host cell autonomous immunity and contributes to virulence.; Authors - Mary Akinyi Nyonda
human	WB, IHC	Cancer Sci; Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance.; Authors - Motoi Fukumoto