GBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 15303-1-AP

Synonyms

Guanylate-binding protein 1, Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1

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Validation Data Gallery

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  WB analysis using 15303-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 15303-1-AP (GBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 15303-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 15303-1-AP (GBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15303-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15303-1-AP (GBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 15303-1-AP

  IP result of anti-GBP1 (IP:15303-1-AP, 4ug; Detection:15303-1-AP 1:600) with human placenta tissue lysate 4000ug.

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  IHC staining of human spleen using 15303-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15303-1-AP (GBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 15303-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15303-1-AP (GBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 15303-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GBP1 antibody 15303-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human placenta tissue, human brain tissue, HUVEC cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 22 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

15303-1-AP targets GBP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP1 fusion protein Ag7562
• Full Name: GTP binding protein 1
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC002666
• Gene Symbol: GBP1
• Gene ID (NCBI): 2633
• RRID: AB_2247448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP.

 What is the molecular weight of GBP1?

The molecular weight of GBP1 is 68kDa.

 What is the cellular localization of GBP1?

GBP1 is predominantly cytosolic, but under certain conditions is farnesylated and associated with intracellular membranes such as the Golgi (PMID: 15937107).

 What is the role of GBP1 in immunity?

GBP1 is induced in response to type I and type II IFN and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica (PMID: 21142871). These anti-pathogenic functions involve the recruitment to vacuolar compartments for the destruction of the pathogen, the assembly of innate immune complexes such as the inflammasome, or the initiation of autophagy.

 What is the role of GBP-1 in colorectal carcinoma?

In colorectal carcinoma (CRC), the presence of tumor-infiltrating T helper type 1 (Th1) cells participates in anti-tumoral responses and an improved clinical outcome (PMID: 17008531, 20927778). IFN promote the Th1 immune response, as well as GBP-1 expression. In CRC, GBP-1 expression acts as a tumor suppressor, where inhibition of tumor growth by GBP-1 is the result of its strong antiangiogenic activity as well as its direct anti-tumorigenic effect on tumor cells and is correlated with a better outcome (PMID: 23042300).

Publications

Species	Application	Title
human	WB,IF	Nat Immunol; Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.; Authors - Michal P Wandel ; KD Validated
human	WB	Nat Cell Biol; LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.; Authors - Gaopeng Li
human	WB	Cell Host Microbe; GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8.; Authors - Michal P Wandel
human	WB,IP	J Med Virol; Guanylate-binding protein 1 inhibits Hantaan virus infection by restricting virus entry; Authors - Tianle Gu
human	WB	Nat Commun; Mechanistic insight into bacterial entrapment by septin cage reconstitution.; Authors - Damián Lobato-Márquez
human	WB,IHC,IF	J Transl Med; Function and mechanism of GBP1 in the development and progression of cervical cancer; Authors - Senyu Wang ; KD Validated