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GBP1 Polyclonal antibody

GBP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15303-1-AP

Synonyms

GBP 1, GBP1, GTP binding protein 1, HuGBP 1



Tested Applications

Positive WB detected inhuman placenta tissue, human brain tissue, HUVEC cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15303-1-AP targets GBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBP1 fusion protein Ag7562
Full Name GTP binding protein 1
Calculated molecular weight 68 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC002666
Gene symbol GBP1
Gene ID (NCBI) 2633
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP.

 What is the molecular weight of GBP1?

The molecular weight of GBP1 is 68kDa.

 What is the cellular localization of GBP1?

GBP1 is predominantly cytosolic, but under certain conditions is farnesylated and associated with intracellular membranes such as the Golgi (PMID: 15937107).

 What is the role of GBP1 in immunity?

GBP1 is induced in response to type I and type II IFN and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica (PMID: 21142871). These anti-pathogenic functions involve the recruitment to vacuolar compartments for the destruction of the pathogen, the assembly of innate immune complexes such as the inflammasome, or the initiation of autophagy.

 What is the role of GBP-1 in colorectal carcinoma?

In colorectal carcinoma (CRC), the presence of tumor-infiltrating T helper type 1 (Th1) cells participates in anti-tumoral responses and an improved clinical outcome (PMID: 17008531, 20927778). IFN promote the Th1 immune response, as well as GBP-1 expression. In CRC, GBP-1 expression acts as a tumor suppressor, where inhibition of tumor growth by GBP-1 is the result of its strong antiangiogenic activity as well as its direct anti-tumorigenic effect on tumor cells and is correlated with a better outcome (PMID: 23042300).


Protocols

Product Specific Protocols
WB protocol for GBP1 antibody 15303-1-APDownload protocol
IHC protocol for GBP1 antibody 15303-1-APDownload protocol
IF protocol for GBP1 antibody 15303-1-APDownload protocol
IP protocol for GBP1 antibody 15303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Aging (Albany NY)

Immune profiling reveals prognostic genes in high-grade serous ovarian cancer.

Authors - Yong Wu
  • KD Validated
humanWB

Arch Virol

Myxovirus resistance protein A inhibits hepatitis C virus replication through JAK-STAT pathway activation.

Authors - Hailong Wang
humanWB, IHC

Cancer Sci

Guanine nucleotide-binding protein 1 is one of the key molecules contributing to cancer cell radioresistance.

Authors - Motoi Fukumoto
humanWB

Oncotarget

PMID: 29137317

Authors - Wu Shuang
humanWB

Cell Host Microbe

GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8.

Authors - Michal P Wandel
humanWB,IF

Nat Immunol

Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.

Authors - Michal P Wandel
  • KD Validated