GBP4 Polyclonal antibody

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  WB analysis using 17746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17746-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17746-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17746-1-AP (GBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17746-1-AP

  IP result of anti-GBP4 (IP:17746-1-AP, 4ug; Detection:17746-1-AP 1:500) with A431 cells lysate 1040 ug.

GBP4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17746-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Mpa2, Guanylate-binding protein 4, Guanine nucleotide-binding protein 4, GTP-binding protein 4, GBP-4

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Tested Applications

• Positive WB detected in: HeLa cells, human placenta tissue, A431 cells, mouse heart tissue
• Positive IP detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17746-1-AP targets GBP4 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GBP4 fusion protein Ag12006
• Full Name: guanylate binding protein 4
• Calculated Molecular Weight: 640 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC070055
• Gene Symbol: GBP4
• Gene ID (NCBI): 115361
• RRID: AB_2109346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PP9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanylate-binding protein 4 (GBP4) is an IFN-inducible GTPase that belongs to the GB1/RHD3 GTPase family. GBP4 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. GBP4 negatively regulates the antiviral response by inhibiting activation of IRF7 transcription factor. The molecular weight of GBP4 is 73 kDa.

Publications

Species	Application	Title
human	WB	mBio; Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation; Authors - Antonia R Bass