GCK Polyclonal antibody

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  WB analysis using 19666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19666-1-AP (GCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19666-1-AP

  IP result of anti-GCK (IP:19666-1-AP, 4ug; Detection:19666-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human liver using 19666-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19666-1-AP (GCK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19666-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19666-1-AP (GCK Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

GCK Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 19666-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HK4, HK IV, Hexokinase-D, Hexokinase-4, Glucokinase

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody is not recommended for WB detection of human samples.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Product Information

19666-1-AP targets GCK in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCK fusion protein Ag8116
• Full Name: glucokinase (hexokinase 4)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001890
• Gene Symbol: GCK
• Gene ID (NCBI): 2645
• RRID: AB_10863656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35557
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Amino acid is a major carbon source for hepatic lipogenesis; Authors - Yilie Liao
mouse	WB	Sci Transl Med; Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes; Authors - Hitoshi Watanabe ; KD Validated
mouse	WB,FC	Nat Commun; Citrullination of glucokinase is linked to autoimmune diabetes.; Authors - Mei-Ling Yang
mouse	WB,IHC	Phytomedicine; Gentiopicroside ameliorates glucose and lipid metabolism in T2DM via targeting FGFR1; Authors - Zhanchi Xu
mouse	WB	PLoS Biol; Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.; Authors - Xuxiao He
mouse	WB	iScience; Mof plays distinct roles in hepatic lipid metabolism under healthy or non-alcoholic fatty liver conditions; Authors - Xinghong Guo