GCLC Polyclonal antibody

GCLC Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12601-1-AP


Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, L02 cells, mouse kidney tissue, mouse lung tissue, Raji cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12601-1-AP targets GCLC in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCLC fusion protein Ag3252
Full Name glutamate-cysteine ligase, catalytic subunit
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC022487
Gene symbol GCLC
Gene ID (NCBI) 2729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).


Product Specific Protocols
WB protocol for GCLC antibody 12601-1-APDownload protocol
IHC protocol for GCLC antibody 12601-1-APDownload protocol
IP protocol for GCLC antibody 12601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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