GFAP Polyclonal antibody

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  WB analysis using 23935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23935-1-AP (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 23935-1-AP

  WB result of GFAP antibody (23935-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells.

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  IP experiment of mouse brain using 23935-1-AP

  IP result of anti-GFAP (IP:23935-1-AP, 4ug; Detection:23935-1-AP 1:20000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 23935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23935-1-AP (GFAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 23935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23935-1-AP (GFAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 23935-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 23935-1-AP (GFAP Antibody) at dilution of 1:50.

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  IF Staining of rat brain using 23935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 23935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 23935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

GFAP antibodies are commonly used as specific markers for astrocytes, enabling identification and analysis of glial cells in the central nervous system for neuroscience and pathology research. Proteintech has an extensive validated GFAP antibodies for WB, IHC, IF-P, IF-Fro, IP, ELISA.

Cat No. 23935-1-AP

Publications(17)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

glial fibrillary acidic protein

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Tested Applications

• Positive WB detected in: U-251 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF-Fro detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 11 publications below

Product Information

23935-1-AP targets GFAP in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, macaque
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFAP fusion protein Ag20853
• Full Name: glial fibrillary acidic protein
• Calculated Molecular Weight: 432 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC013596
• Gene Symbol: GFAP
• Gene ID (NCBI): 2670
• RRID: AB_2879367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14136
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central  nervous system (CNS). GFAP is one of the  main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker.  However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations  present in affected individuals are thought to be gain-of-function. 

• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular  processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various  neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. 

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916),  which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Publications

Species	Application	Title
mouse	IF	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	IF	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng
mouse	IF	Sci Rep; Administration of molecular hydrogen during pregnancy improves behavioral abnormalities of offspring in a maternal immune activation model.; Authors - Kenji Imai
mouse	WB	Invest Ophthalmol Vis Sci; High Intraocular Concentration of Fibrinogen Regulates Retinal Function Via the ICAM-1 Pathway; Authors - Yasuyuki Sotani

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Autumn (Verified Customer) (02-08-2024)

Works wonderfully!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: patient derived glioblastoma stem-like cells