ChromoTek GFP VHH, recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein

Specificity

CFP, eGFP, AcGFP, eYFP

Applications

ELISA, IF, IP

Conjugate

Unconjugated

Publications

23

Cat no : gt

Synonyms

GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)

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Validation Data Gallery

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  Structure of GFP-VHH.

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Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein

• Applications: ELISA, IF, IP
• Clonality: VHH
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
• Molecular Weight: 13.9 kDa
• Conjugate: Unconjugated
• Storage Buffer: 25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.

Publications

Application	Title
IF	ACS Chem Biol; A simple method for GFP- and RFP-based dual color single-molecule localization microscopy.; Authors - Evgenia Platonova
ELISA	Int J Mol Sci; Applying Unconventional Secretion in Ustilago maydis for the Export of Functional Nanobodies.; Authors - Marius Terfrüchte
WB	Cell Rep; Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes.; Authors - Sivakami Mylvaganam
IF	Nat Protoc; Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.; Authors - Sumit K Chaturvedi
IP	ACS Sens; Litmus-Body: A Molecularly Targeted Sensor for Cell-Surface pH Measurements.; Authors - Joe Chin-Hun Kuo
	Bio Protoc; Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes.; Authors - Ammarah Tariq