ChromoTek GFP VHH, recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein


GFP and GFP derivates







Cat no : gt


GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)

Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein

DescriptionAlpaca anti-GFP VHH, purified recombinant binding protein for extraordinary stable & reliable binding

• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

• Fulfills highest requirements for antibody validation

• Structure and function are characterized

ApplicationsSuitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Specificity/TargetCFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
Type Nanobody
Affinity (KD) Dissociation constant KD of 1 pM
Molecular Weight13.9 kDa
Storage Buffer25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.
BackgroundGreen Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This Nanobody is an alpaca recombinant VHH raised against GFP, reactive to a variety of GFP derivates, including CFP, eGFP, yeast eGFP, Clover, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, YPET, Citrine and eCitrine and more.
For a full list of specificities see: Fluorescent_Specificity_by_Nano-Trap_190222.pdf (



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