GLAST Polyclonal antibody

GLAST Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20785-1-AP

Synonyms

EA6, EAAT1, GLAST, GLAST 1, GLAST1, SLC1A3



Tested Applications

Positive WB detected inNeuro-2a cells, C6 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20785-1-AP is GLAST Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLAST fusion protein Ag14177
Full Name solute carrier family 1 (glial high affinity glutamate transporter), member 3
Calculated molecular weight 542 aa, 60 kDa
Observed molecular weight 50-55 kDa, 90-100 kDa
GenBank accession numberBC037310
Gene symbol SLC1A3
Gene ID (NCBI) 6507
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)

Protocols

Product Specific Protocols
WB protocol for GLAST antibody 20785-1-APDownload protocol
IHC protocol for GLAST antibody 20785-1-APDownload protocol
IP protocol for GLAST antibody 20785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Ginsenoside Rb1 prevents MPTP-induced changes in hippocampal memory via regulation of the α-synuclein/PSD-95 pathway.

Authors - Shaogang Qu
mouseIHC

Neurosci Bull

Expression Patterns of Inducible Cre Recombinase Driven by Differential Astrocyte-Specific Promoters in Transgenic Mouse Lines.

Authors - Neng-Yuan Hu
mouseWB

Cell Death Dis

Functional validation of a human GLUD2 variant in a murine model of Parkinson's disease.

Authors - Wenlong Zhang

Biochem Biophys Res Commun

Exosomal and extracellular HMGB1 have opposite effects on SASH1 expression in rat astrocytes and glioma C6 cells.

Authors - Chao Ma
ratIF

Brain Res

iTRAQ-based proteomic analysis after mesenchymal stem cell line transplantation for ischemic stroke.

Authors - Shingo Mitaki
ratWB

Brain Res Bull

PMID: 32621862

Authors - Fang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph = 6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina and brain at 5dpf
GLAST Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina and brain at 5dpf (Cat no:20785-1-AP)
FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - 0.1M Tris-HCl ph9

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina/brain
GLAST Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina/brain (Cat no:20785-1-AP)