GLI3-Specific Polyclonal antibody

GLI3-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 19949-1-AP

Synonyms

ACLS, GCPS, GLI family zinc finger 3, GLI3, GLI3-Specific, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3

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Validation Data Gallery

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  WB analysis of human placenta using 19949-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19949-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19949-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19949-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19949-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19949-1-AP (GLI3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 19949-1-AP

  IP result of anti-GLI3-Specific (IP:19949-1-AP, 4ug; Detection:19949-1-AP 1:300) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human testis using 19949-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human colon using 19949-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.

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  IHC staining of human colon using 19949-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 19949-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19949-1-AP (GLI3-Specific Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 19949-1-AP

  Immunofluorescent analysis of HepG2 cells using 19949-1-AP (GLI3-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HepG2 using 19949-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GLI3-Specific antibody (19949-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below

Product Information

19949-1-AP targets GLI3-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GLI family zinc finger 3
• Calculated Molecular Weight: 170 kDa
• Observed Molecular Weight: 190 kDa, 83-86 kDa
• GenBank Accession Number: NM_000168
• Gene Symbol: GLI3
• Gene ID (NCBI): 2737
• RRID: AB_11182281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Publications

Species	Application	Title
mouse	WB	Nat Commun; mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.; Authors - Bo Yan
mouse	WB	JCI Insight; Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via Sonic Hedgehog signaling.; Authors - Hairong Shi
mouse	WB	Acta Pharmacol Sin; 1-Indanone retards cyst development in ADPKD mouse model by stabilizing tubulin and down-regulating anterograde transport of cilia; Authors - Xiao-Wei Li
mouse	WB	PLoS Genet; Depletion of Ric-8B leads to reduced mTORC2 activity.; Authors - Maíra H Nagai
mouse	WB	Sci Rep; LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration.; Authors - Yuqin Men
mouse	WB	Int J Biol Sci; MicroRNA-223 restricts liver fibrosis by inhibiting the TAZ-IHH-GLI2 and PDGF signaling pathways via the crosstalk of multiple liver cell types.; Authors - Xiaolin Wang