GLI3-Specific Polyclonal antibody

GLI3-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19949-1-AP

Synonyms

ACLS, GCPS, GLI family zinc finger 3, GLI3, PAP A, PAPA, PAPA1, PAPB, PHS, PPDIV, Zinc finger protein GLI3



Tested Applications

Positive WB detected inhuman placenta tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

19949-1-AP targets GLI3-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GLI family zinc finger 3
Calculated molecular weight 170 kDa
Observed molecular weight 190 kDa, 83-86 kDa
GenBank accession numberNM_000168
Gene symbol GLI3
Gene ID (NCBI) 2737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLI3 belongs to the GLI C2H2-type zinc-finger protein family. GLI3 plays a role in limb and brain development. GLI3 is implicated in the transduction of SHH signal. Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS). Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB). Defects in GLI3 are a cause of type IV preaxial polydactyly. Defects in GLI3 are the cause of acrocallosal syndrome (ACS). The antibody is specific to GLI3. At the molecular level, Gli3 is translated into a 190-kDa transcriptional activator (Gli3-190) that undergoes proteolytic processing into a truncated 83-kDa repressor (Gli3-83) lacking C-terminal activation domains. (PMID: 16705181)

Protocols

Product Specific Protocols
WB protocol for GLI3-Specific antibody 19949-1-APDownload protocol
IHC protocol for GLI3-Specific antibody 19949-1-APDownload protocol
IF protocol for GLI3-Specific antibody 19949-1-APDownload protocol
IP protocol for GLI3-Specific antibody 19949-1-APDownload protocol
FC protocol for GLI3-Specific antibody 19949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

Regulation of Survivin Isoform Expression by GLI Proteins in Ovarian Cancer.

Authors - Diana Trnski
mouseWB

PLoS Genet

Depletion of Ric-8B leads to reduced mTORC2 activity.

Authors - Maíra H Nagai
humanWB

Biochim Biophys Acta

GSK3β and Gli3 play a role in activation of Hedgehog-Gli pathway in human colon cancer - Targeting GSK3β downregulates the signaling pathway and reduces cell proliferation.

Authors - Diana Trnski
humanWB

Acta Biochim Biophys Sin (Shanghai)

Effects of baicalein on pancreatic cancer stem cells via modulation of sonic Hedgehog pathway.

Authors - Libin Song
mouseWB

Sci Rep

LKB1 Regulates Cerebellar Development by Controlling Sonic Hedgehog-mediated Granule Cell Precursor Proliferation and Granule Cell Migration.

Authors - Yuqin Men
mouseWB

Nat Commun

mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.

Authors - Bo Yan