|Positive WB detected in||HEK-293 cells, human brain tissue, human heart tissue, K-562 cells, mouse brain tissue, mouse kidney tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human brain tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:400|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20170-1-AP is KGA-Specific Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KGA-Specific fusion protein Ag14002|
|Calculated molecular weight||669 aa, 73 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC038507|
|Gene ID (NCBI)||2744|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.
|Product Specific Protocols|
|WB protocol for KGA-Specific antibody 20170-1-AP||Download protocol|
|IHC protocol for KGA-Specific antibody 20170-1-AP||Download protocol|
|IF protocol for KGA-Specific antibody 20170-1-AP||Download protocol|
|IP protocol for KGA-Specific antibody 20170-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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