KGA-Specific Polyclonal antibody

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  WB analysis of A549 using 20170-1-AP

  WB result of KGA-Specific antibody (20170-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KGA-Specific transfected A549 cells.

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  WB analysis of HEK-293 using 20170-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis using 20170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20170-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20170-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20170-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20170-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20170-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20170-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20170-1-AP (KGA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20170-1-AP

  IP result of anti-KGA-Specific (IP:20170-1-AP, 4ug; Detection:20170-1-AP 1:8000) with HEK-293 cells lysate 1440 ug.

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  IP experiment of HFF using 20170-1-AP

  IP result of anti-KGA-Specific (IP:20170-1-AP, 4ug; Detection:20170-1-AP 1:8000) with HFF cells lysate 1000 ug.

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  IHC staining of human kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 20170-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20170-1-AP (KGA-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of hela using 20170-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed hela cells using KGA-Specific antibody (20170-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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  IF Staining of HEK-293 using 20170-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20170-1-AP (KGA-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 20170-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KGA-Specific antibody (20170-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

KGA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20170-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

GLS, KGA, GLS1, GAM, GAC

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, human heart tissue, human brain tissue, K-562 cells, mouse brain tissue, A549 cells, HeLa cells, rat brain tissue, HFF cells, MRC-5 cells
• Positive IP detected in: HEK-293 cells, HFF cells
• Positive IHC detected in: human kidney tissue, human brain tissue, mouse brain tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hela cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

20170-1-AP targets KGA-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KGA-Specific fusion protein Ag14002
• Full Name: glutaminase
• Calculated Molecular Weight: 669 aa, 73 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC038507
• Gene Symbol: GLS
• Gene ID (NCBI): 2744
• RRID: AB_10665373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94925
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

Publications

Species	Application	Title
mouse	WB	Nat Med; Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.; Authors - Daniel Herranz
mouse	WB	Cell Stem Cell; CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.; Authors - Gianluca L Russo
human	WB	Blood; Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.; Authors - Nathalie Jacque
	WB	Elife; Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor; Authors - Carolyn M Jablonowski
rat	CoIP, WB	Int J Mol Sci; Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.; Authors - Małgorzata Beręsewicz-Haller
human	WB,IHC	EBioMedicine; Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.; Authors - Binghua Li