KGA-Specific Polyclonal antibody

KGA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20170-1-AP

Synonyms

AAD20, GLS, GLS1, GLSK, glutaminase, K glutaminase, KGA, KGA-specific, KIAA0838, L glutamine amidohydrolase



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, human heart tissue, K-562 cells, mouse brain tissue, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20170-1-AP is KGA-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KGA-Specific fusion protein Ag14002
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC038507
Gene symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

Protocols

Product Specific Protocols
WB protocol for KGA-Specific antibody 20170-1-APDownload protocol
IHC protocol for KGA-Specific antibody 20170-1-APDownload protocol
IF protocol for KGA-Specific antibody 20170-1-APDownload protocol
IP protocol for KGA-Specific antibody 20170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
human

RNA

CFIm25 regulates glutaminase alternative terminal exon definition to modulate miR-23 function.

Authors - Chioniso P Masamha
mouseWB

Cell Stem Cell

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Authors - Gianluca L Russo
humanWB

eNeuro

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

Authors - Kalpana D Acharya
ratCoIP, WB

Int J Mol Sci

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Authors - Małgorzata Beręsewicz-Haller
humanWB

AIDS Res Hum Retroviruses

Evidence for altered glutamine metabolism in HIV-1 infected primary human CD4+ T cells.

Authors - Andrea Hegedus