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KGA-Specific Polyclonal antibody

KGA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20170-1-AP

Synonyms

AAD20, GLS, GLS1, GLSK, glutaminase, K glutaminase, KGA, KGA-specific, KIAA0838, L glutamine amidohydrolase



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, human heart tissue, human brain tissue, K-562 cells, mouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20170-1-AP targets KGA-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KGA-Specific fusion protein Ag14002
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC038507
Gene symbol GLS
Gene ID (NCBI) 2744
RRIDAB_10665373
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

Protocols

Product Specific Protocols
WB protocol for KGA-Specific antibody 20170-1-APDownload protocol
IHC protocol for KGA-Specific antibody 20170-1-APDownload protocol
IF protocol for KGA-Specific antibody 20170-1-APDownload protocol
IP protocol for KGA-Specific antibody 20170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Med

Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia.

Authors - Daniel Herranz
mouseWB

Cell Stem Cell

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Authors - Gianluca L Russo
humanWB

Blood

Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.

Authors - Nathalie Jacque
ratCoIP, WB

Int J Mol Sci

Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Authors - Małgorzata Beręsewicz-Haller
humanWB,IHC

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanWB

J Genet Genomics

A facile and sensitive method of quantifying glutaminase binding to its inhibitor CB-839 in tissues.

Authors - Yicheng Chen