GLS Polyclonal antibody

GLS Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 29519-1-AP

Synonyms

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KIAA0838, L glutamine amidohydrolase

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Validation Data Gallery

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  WB analysis using 29519-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 29519-1-AP (GLS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 29519-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using GLS antibody (29519-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Hela cells, MCF-7 cells, SH-SY5Y cells
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

29519-1-AP targets GLS in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLS fusion protein Ag31167
• Full Name: glutaminase
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 58 kDa, 65 kDa
• GenBank Accession Number: NM_014905
• Gene Symbol: GLS
• Gene ID (NCBI): 2744
• RRID: AB_2918320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

Publications

Species	Application	Title
mouse	WB	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
human	WB	Front Oncol; ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer; Authors - Xingyu Zhu
human	WB	Front Pharmacol; A novel method for generating glutamatergic SH-SY5Y neuron-like cells utilizing B-27 supplement; Authors - Emily-Rose Martin
rat	WB	Gen Comp Endocrinol; Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats; Authors - Dong Li
human,mouse	WB,IHC,IF	Mol Carcinog; Glutaminase potentiates the glycolysis in esophageal squamous cell carcinoma by interacting with PDK1; Authors - Guangzhao Zhu