GLS Polyclonal antibody

GLS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

Unconjugated

Cat no : 29519-1-AP

Synonyms

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KIAA0838, L glutamine amidohydrolase

---

Validation Data Gallery

• 

  WB analysis using 29519-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 29519-1-AP (GLS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: COLO 320 cells, Hela cells, MCF-7 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Product Information

29519-1-AP targets GLS in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLS fusion protein Ag31167
• Full Name: glutaminase
• Calculated molecular weight: 73 kDa
• Observed molecular weight: 58 kDa, 65 kDa
• GenBank accession number: NM_014905
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.