Tested Applications
| Positive WB detected in | COLO 320 cells, A549 cells, Hela cells, MCF-7 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29519-1-AP targets KGA/GAC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31167 Product name: Recombinant human GLS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-140 aa of NM_014905 Sequence: MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEGKELVASGENKIKQGLLPSLE Predict reactive species |
| Full Name | glutaminase |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 58 kDa, 65 kDa |
| GenBank Accession Number | NM_014905 |
| Gene Symbol | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_2918320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94925 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KGA/GAC antibody 29519-1-AP | Download protocol |
| WB protocol for KGA/GAC antibody 29519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KGA/GAC Polyclonal antibody (Cat# 29519-1-AP) has accumulated 23 citations published in journals such as Nature Metabolism, Advanced Materials, Cell Reports, Cancer Letters, Biomaterials, Free Radical Biology and Medicine, and Journal of Biological Chemistry. Associated research fields include glutamine metabolism, cancer biology (colorectal, gastric, NSCLC, glioblastoma), ferroptosis, cuproptosis, cartilage regeneration, gut microbiome, mitochondrial function, neuroprotection, and environmental toxicology. The primary application is Western blot, supplemented by IHC and IF.
| Species | Application | Title |
|---|---|---|
Adv Mater Mechano-Iontronic Hydrogels Generating Biomimetic Endogenous Bioelectricity for Promoting Cartilage Regeneration. | ||
J Adv Res SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels | ||
Biomaterials Topology scaffolds-enhanced paracrine of BMSCs through mechanotransduction-related metabolism reprogramming for burn wounds healing | ||
Environ Sci Technol Benzo(a)anthracene Targeting SLC1A5 to Synergistically Enhance PAH Mixture Toxicity | ||
Cell Death Dis ΔNp63α drives serine synthesis to promote carboplatin resistance in NSCLC. |
