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- KD/KO Validated
KGA/GAC Monoclonal antibody
KGA/GAC Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
human, pig, mouse, rat
WB, IHC, IF, ELISA
Cat no : 66265-1-Ig
Validation Data Gallery
|Positive WB detected in||NIH/3T3 cells, rat liver tissue, fetal human brain tissue, U-251 cells, HEK-293 cells|
|Positive IHC detected in||human gliomas tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, HepG2 cells, L02 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 4 publications below|
|IF||See 2 publications below|
66265-1-Ig targets KGA/GAC in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
|Tested Reactivity||human, pig, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||KGA/GAC fusion protein Ag24227|
|Calculated molecular weight||669 aa, 73 kDa|
|Observed molecular weight||58 kDa, 65 kDa|
|GenBank accession number||BC038507|
|Gene ID (NCBI)||2744|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
|Product Specific Protocols|
|WB protocol for KGA/GAC antibody 66265-1-Ig||Download protocol|
|IHC protocol for KGA/GAC antibody 66265-1-Ig||Download protocol|
|IF protocol for KGA/GAC antibody 66265-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.
Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
Int J Oncol
Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.
Oxid Med Cell Longev
Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages.
Prostate cancer cells demonstrate unique metabolism and substrate adaptability acutely after androgen deprivation therapy
J Mol Cell Cardiol
Glutamine uptake and catabolism is required for myofibroblast formation and persistence
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Longlong (Verified Customer) (03-01-2022)
I'm working on a glutaminase project. Great western blot results and fantastic consistency