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  • KD/KO Validated

KGA/GAC Monoclonal antibody

KGA/GAC Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


human, pig, mouse, rat







Cat no : 66265-1-Ig


AAD20, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA, KIAA0838, L glutamine amidohydrolase

Tested Applications

Positive WB detected inNIH/3T3 cells, rat liver tissue, fetal human brain tissue, U-251 cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HepG2 cells, L02 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66265-1-Ig targets KGA/GAC in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen KGA/GAC fusion protein Ag24227
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight 58 kDa, 65 kDa
GenBank accession numberBC038507
Gene symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.


Product Specific Protocols
WB protocol for KGA/GAC antibody 66265-1-IgDownload protocol
IHC protocol for KGA/GAC antibody 66265-1-IgDownload protocol
IF protocol for KGA/GAC antibody 66265-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
  • KO Validated

Cell Rep

Glutaminase 1 deficiency confined in forebrain neurons causes autism spectrum disorder-like behaviors

Authors - Chenhui Ji

Oxid Med Cell Longev

Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages.

Authors - Yifan Feng

Int J Oncol

Cryptotanshinone suppresses tumorigenesis by inhibiting lipogenesis and promoting reactive oxygen species production in KRAS‑activated pancreatic cancer cells.

Authors - Tokio Terado


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sarah-Eve (Verified Customer) (06-02-2023)

Works well

  • Applications: Western Blot

Longlong (Verified Customer) (03-01-2022)

I'm working on a glutaminase project. Great western blot results and fantastic consistency

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human AML cell lines
KGA/GAC Antibody Western Blot validation (1:1000 dilution) in human AML cell lines (Cat no:66265-1-Ig)