GLUD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLUD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 14299-1-AP

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1

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Validation Data Gallery

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  WB analysis using 14299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14299-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14299-1-AP

  IP Result of anti-GLUD1 (IP:14299-1-AP, 4ug; Detection:14299-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, HepG2 cells, L02 cells, PC-3 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 28 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14299-1-AP targets GLUD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLUD1 fusion protein Ag5694
• Full Name: glutamate dehydrogenase 1
• Calculated molecular weight: 61 kDa
• Observed molecular weight: 45-55 kDa
• GenBank accession number: BC040132
• Gene symbol: GLUD1
• Gene ID (NCBI): 2746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Publications

Species	Application	Title
		Science; Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.; Authors - Jessica B Spinelli
human	WB	Cell; Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.; Authors - Wen Yang
human	WB	Cancer Cell; Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.; Authors - Meng Li
		Cell Metab; Differential Glutamate Metabolism in Proliferating and Quiescent Mammary Epithelial Cells.; Authors - Jonathan L Coloff
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang ; KD Validated