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- KD/KO Validated
GLUD1 Polyclonal antibody
GLUD1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 14299-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse lung tissue, HepG2 cells, L02 cells, PC-3 cells, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human gliomas tissue, human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 28 publications below|
|IHC||See 3 publications below|
|IF||See 1 publications below|
|IP||See 1 publications below|
14299-1-AP targets GLUD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GLUD1 fusion protein Ag5694|
|Full Name||glutamate dehydrogenase 1|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||45-55 kDa|
|GenBank accession number||BC040132|
|Gene ID (NCBI)||2746|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.
|Product Specific Protocols|
|WB protocol for GLUD1 antibody 14299-1-AP||Download protocol|
|IHC protocol for GLUD1 antibody 14299-1-AP||Download protocol|
|IP protocol for GLUD1 antibody 14299-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.
Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.
Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.
Differential Glutamate Metabolism in Proliferating and Quiescent Mammary Epithelial Cells.
Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.