• Featured Product
  • KD/KO Validated

GLUD1 Polyclonal antibody

GLUD1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14299-1-AP

Synonyms

GDH, GDH 1, GDH1, GLUD, GLUD1, glutamate dehydrogenase 1



Tested Applications

Positive WB detected inL02 cells, HeLa cells, HepG2 cells, PC-3 cells, mouse liver tissue, mouse lung tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14299-1-AP targets GLUD1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLUD1 fusion protein Ag5694
Full Name glutamate dehydrogenase 1
Calculated molecular weight 61 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC040132
Gene symbol GLUD1
Gene ID (NCBI) 2746
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Protocols

Product Specific Protocols
WB protocol for GLUD1 antibody 14299-1-APDownload protocol
IHC protocol for GLUD1 antibody 14299-1-APDownload protocol
IP protocol for GLUD1 antibody 14299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Authors - Alex J Bott
humanWB

Aging (Albany NY)

The cross-talk between DDR1 and STAT3 promotes the development of hepatocellular carcinoma.

Authors - Ye Lin
humanWB

J Ethnopharmacol

Effects of Dahuang zhechong pill on doxorubicin-resistant SMMC-7721 xenografts in mice.

Authors - Li Wu
humanWB

Amino Acids

PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Authors - Chunhong Cui
WB

PLoS One

Replication of the Shrimp Virus WSSV Depends on Glutamate-Driven Anaplerosis.

Authors - Chun-Yuan Li

J Alzheimers Dis

Cerebral Level of vGlut1 is Increased and Level of Glycine is Decreased in TgSwDI Mice.

Authors - Timmer Nienke M NM