GLUD1 Polyclonal antibody

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  WB analysis using 14299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14299-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14299-1-AP (GLUD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14299-1-AP

  IP result of anti-GLUD1 (IP:14299-1-AP, 4ug; Detection:14299-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 14299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14299-1-AP (GLUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using GLUD1 antibody (14299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GLUD1 antibody (14299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

GLUD1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 14299-1-AP

Publications(53)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA

Synonyms

EC:1.4.1.3, GDH, GDH 1, GDH1, GLUD

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, HepG2 cells, L02 cells, PC-3 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human breast cancer tissue, human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF-Fro detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 48 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

14299-1-AP targets GLUD1 in WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLUD1 fusion protein Ag5694
• Full Name: glutamate dehydrogenase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC040132
• Gene Symbol: GLUD1
• Gene ID (NCBI): 2746
• RRID: AB_2110515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00367
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.

Publications

Species	Application	Title
		Science; Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.; Authors - Jessica B Spinelli
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human	WB	Cell; Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.; Authors - Wen Yang
human	WB	Cancer Cell; Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.; Authors - Meng Li
mouse	WB	Nat Metab; Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis; Authors - Grace Park