GMEB1-Specific Recombinant antibody

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  WB analysis using 84448-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84448-1-RR (GMEB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84448-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GMEB1-Specific Recombinant antibody (84448-1-RR, Clone:241447C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

GMEB1-Specific {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84448-1-RR

Clone No.241447C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

GMEB1, GMEB-1, GMEB 1, Glucocorticoid modulatory element-binding protein 1, DNA-binding protein p96PIF

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, Daudi cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84448-1-RR targets GMEB1-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: glucocorticoid modulatory element binding protein 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: NM_006582
• Gene Symbol: GMEB1
• Gene ID (NCBI): 10691
• RRID: AB_3671974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMEB1 is a trans-acting factor that binds to glucocorticoid modulatory elements (GME), acting in estrogen receptor-regulated pathway GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to function in hormone-dependent cancer growth. It associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication.