GOLGA4 Polyclonal antibody

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  WB analysis using 55207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55207-1-AP (GOLGA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GOLGA4 Polyclonal Antibody for WB, ELISA

Cat No. 55207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MU RMS 40.18, Golgin subfamily A member 4, Golgin 245, GOLG, GCP2

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Tested Applications

• Positive WB detected in: T-47D cells, U-118 MG cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55207-1-AP targets GOLGA4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: golgi autoantigen, golgin subfamily a, 4
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 260-270 kDa
• GenBank Accession Number: NM_002078
• Gene Symbol: GOLGA4
• Gene ID (NCBI): 2803
• RRID: AB_3670242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13439
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA4, also known as Golgi matrix protein, is a member of the Golgin family and is mainly localized in the Golgi apparatus. It is involved in vesicle transport at the Golgi level and may play a role in the transport of GPI-anchored proteins from the Golgi network. GOLGA4 is expressed in a variety of tissues, especially in the testis, but its specific function in spermatogenesis and male fertility is not fully understood. Studies have shown that GOLGA4 knockout male mice are still fertile, and the testis and sperm morphology are normal, indicating that GOLGA4 is not essential for mouse spermatogenesis and male fertility (PMID: 32736686).