GPR45 Recombinant antibody

• 

  WB analysis using 84587-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84587-1-RR (GPR45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 84587-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84587-1-RR (GPR45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84587-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84587-1-RR (GPR45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR45 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 84587-1-RR

Clone No.241776D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PSP24(ALPHA), PSP24 alpha, PSP24 1, PSP24, G protein coupled receptor 45

---

Tested Applications

• Positive WB detected in: mouse brain tissue, unboiled mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84587-1-RR targets GPR45 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 45
• Calculated Molecular Weight: 370 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC066879
• Gene Symbol: GPR45
• Gene ID (NCBI): 11250
• RRID: AB_3672083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y5Y3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR45 (G-protein coupled receptor 45) is a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPR45 may function in the central nervous system.