GPSM2 Polyclonal antibody

GPSM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26798-1-AP

Synonyms

GPSM2, LGN, Mosaic protein LGN, Pins

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Validation Data Gallery

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  WB analysis using 26798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26798-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26798-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26798-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 26798-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26798-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26798-1-AP (GPSM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26798-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26798-1-AP (GPSM2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 26798-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 26798-1-AP (GPSM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, HEK-293T cells, mouse liver tissue, HEK-293 cells
• Positive IHC detected in: human placenta tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26798-1-AP targets GPSM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPSM2 fusion protein Ag25195
• Full Name: G-protein signaling modulator 2 (AGS3-like, C. elegans)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-77 kDa
• GenBank Accession Number: BC027732
• Gene Symbol: GPSM2
• Gene ID (NCBI): 29899
• RRID: AB_2880640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.