GPSN2 Polyclonal antibody

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  WB analysis using 30047-1-AP

  HeLa cells lysates were subjected to SDS PAGE followed by western blot with 30047-1-AP (GPSN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 30047-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using GPSN2 antibody (30047-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

GPSN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Trans-2,3-enoyl-CoA reductase, TER, TECR, Synaptic glycoprotein SC2, SC2

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30047-1-AP targets GPSN2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPSN2 fusion protein Ag32598
• Full Name: glycoprotein, synaptic 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC002425
• Gene Symbol: GPSN2
• Gene ID (NCBI): 9524
• RRID: AB_3086219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZ01
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycoprotein synaptic 2 (GPSN2), also known as TER and TECR, functions as a trans-2,3-enoyl-CoA reductase and reduces long and very long chain fatty acid trans-2,3-enoyl-CoA substrates (PMID: 12482854).