Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | rat eye tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13947-1-AP targets GPX3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5054 Product name: Recombinant human GPX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-72 aa of BC050378 Sequence: MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASY Predict reactive species |
| Full Name | glutathione peroxidase 3 (plasma) |
| Calculated Molecular Weight | 226 aa, 26 kDa |
| Observed Molecular Weight | 20-27 kDa |
| GenBank Accession Number | BC050378 |
| Gene Symbol | GPX3 |
| Gene ID (NCBI) | 2878 |
| RRID | AB_3085426 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22352 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GPX3 antibody 13947-1-AP | Download protocol |
| IHC protocol for GPX3 antibody 13947-1-AP | Download protocol |
| WB protocol for GPX3 antibody 13947-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPX3 Polyclonal antibody (Cat# 13947-1-AP) has 24 citations across journals including Theranostics, Biomaterials, Cell Reports, Free Radical Biology and Medicine, Molecular Therapy, iScience, eLife, and Scientific Reports. Research fields span oxidative stress, ferroptosis, cancer (TNBC, ccRCC, lung adenocarcinoma), diabetic nephropathy, spinal cord injury, cardiomyopathy, hearing loss, endometriosis, autoimmune encephalitis, and pulmonary arterial hypertension, with applications primarily in WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Theranostics TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress. | ||
Biomaterials Modeling calcific aortic valve disease with engineered human valve tissues identifies SAMHD1 as a therapeutic target. | ||
Int J Biol Sci Immune Cell Infiltration and Kynurenine Pathway Activation Define Early Injury and Progression in Diabetic Nephropathy. | ||
Cell Commun Signal GPX3 promotes cisplatin resistance in TNBC by manipulating ROS-TGFB1-ZEB2
| ||
Mol Ther Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes. | ||
Free Radic Biol Med GPX3 activates autophagy to protect cochlear spiral ganglion neurons from injury in age-related hearing loss. |
