• Featured Product
  • KD/KO Validated

GPX3 Polyclonal antibody

GPX3 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13947-1-AP

Synonyms

Glutathione peroxidase 3, GPx 3, GPx P, GPX3, GPXP, GSHPx 3, GSHPx P, Plasma glutathione peroxidase



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, human placenta tissue
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13947-1-AP targets GPX3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPX3 fusion protein Ag5054
Full Name glutathione peroxidase 3 (plasma)
Calculated molecular weight 226 aa, 26 kDa
Observed molecular weight 20-27 kDa
GenBank accession numberBC050378
Gene symbol GPX3
Gene ID (NCBI) 2878
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).

Publications

SpeciesApplicationTitle
mouseWB

Mol Carcinog

MARVELD1 interacting with catalase regulates reactive oxygen species metabolism and mediates the sensitivity to chemotherapeutic drugs in epithelial tumors of the reproductive system.

Authors - Chunbin Zhang
humanIF

Mol Vis

Long noncoding RNA glutathione peroxidase 3-antisense inhibits lens epithelial cell apoptosis by upregulating glutathione peroxidase 3 expression in age-related cataract.

Authors - Yuanyuan Tu
chickenWB

J Cell Physiol

Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes.

Authors - Yafan Gong
  • KD Validated
mouseWB

Theranostics

TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress.

Authors - Kailiang Zhou